LOCUS       sequence-116858-         825 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Golgi TGN, Excitation: 568, Emission: 592.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 825)
  TITLE     Davidson lab - mApple constructs
REFERENCE   2  (bases 1 to 825)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mApple-SiT-N-15
FEATURES             Location/Qualifiers
     source          1..825
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      227..236
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     646..665
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 tgaaccgtca gatccgctag cgctaccgga ctcagatctc gagctcgcca ccatgattca
       61 caccaacctg aagaaaaagt tcagctgctg cgtcctggtc tttcttctgt ttgcagtcat
      121 ctgtgtgtgg aaggaaaaga agaaagggag ttactatgat tcctttaaat tgcaaaccaa
      181 ggaattcctg cagtcgacgg taccgcgggc ccgggatcca ccggtcgcca ccatggtgag
      241 caagggcgag gagaataaca tggccatcat caaggagttc atgcgcttca aggtgcacat
      301 ggagggctcc gtgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta
      361 cgaggccttt cagaccgcta agctgaaggt gaccaagggt ggccccctgc ccttcgcctg
      421 ggacatcctg tcccctcagt tcatgtacgg ctccaaggtc tacattaagc acccagccga
      481 catccccgac tacttcaagc tgtccttccc cgagggcttc aggtgggagc gcgtgatgaa
      541 cttcgaggac ggcggcatta ttcacgttaa ccaggactcc tccctgcagg acggcgtgtt
      601 catctacaag gtgaagctgc gcggcaccaa cttcccctcc gacggccccg taatgcagaa
      661 gaagaccatg ggctgggagg cctccgagga gcggatgtac cccgaggacg gcgccctgaa
      721 gagcgagatc aagaagaggc tgaagctgaa ggacggcggc cactacgccg ccgaggtcaa
      781 gaccacctac aaggccaaga agcccgtgca gctgcccggc gccta
//
