LOCUS       sequence-116860-        1064 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Nucleus, Excitation: 590, Emission: 649.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1064)
  TITLE     Davidson Red fluorescent proteins
REFERENCE   2  (bases 1 to 1064)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: mPlum-Nucleus-7
FEATURES             Location/Qualifiers
     source          1..1064
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             62..739
                     /codon_start=1
                     /product="far-red monomeric derivative of DsRed fluorescent
                     protein (Wang et al., 2004)"
                     /label=mPlum
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEVIKEFMRFKEHMEGSVNGHEFEIEGEGEGRPYEGTQTA
                     RLKVTKGGPLPFAWDILSPQIMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGG
                     VVTVTQDSSLQDGEFIYKVKVRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALKGEMK
                     MRLRLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRH
                     STGA"
     primer_bind     463..482
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     657..680
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     CDS             764..784
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             788..808
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             812..832
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 tgggnnntta gcagagctgg ttngtgaacc gtcagatccg ctagcgctac cggtggctac
       61 catggtgagc aagggcgagg aggtcatcaa ggagttcatg cgcttcaagg agcacatgga
      121 gggctccgtg aacggccacg agttcgagat cgagggcgag ggcgagggcc gcccctacga
      181 gggcacccag accgccaggc tgaaggtgac caagggtggc cccctgccct tcgcctggga
      241 catcctgtcc cctcagatca tgtacggctc caaggcctac gtgaagcacc ccgccgacat
      301 ccccgactac ttgaagctgt ccttccccga gggcttcaag tgggagcgcg tgatgaactt
      361 cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc ctgcaggacg gcgagttcat
      421 ctacaaggtg aaggtgcgcg gcaccaactt cccctccgac ggccccgtaa tgcagaagaa
      481 gaccatgggc tgggaggcct cctccgagcg gatgtacccc gaggacggcg ccctgaaggg
      541 cgagatgaag atgaggctga ggctgaagga cggcggccac tacgacgccg aggtcaagac
      601 cacctacatg gccaagaagc ccgtgcagct gcccggcgcc tacaagaccg acatcaagct
      661 ggacatcacc tcccacaacg aggactacac catcgtggaa cagtacgagc gcgccgaggg
      721 ccgccactcc accggcgcct ccggactcag atctcgagct gatccaaaaa agaagagaaa
      781 ggtagatcca aaaaagaaga gaaaggtaga tccaaaaaag aagagaaagg taggatccac
      841 cggatctaga taactgatca taatcagcca taccacattt gtagaggttt tacttgcttt
      901 aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa ttgttgnttg
      961 ttaacttgtt tattgcanct tataatggtt acaaatnaan gcaatancat cacaaatttc
     1021 acaaataaag catttttttc actgcattct antngtggtt tngt
//
