LOCUS       sequence-116868-         584 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Plasmid encoding multiple cloning site, rybozyme and IRES-CAS9..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 584)
  AUTHORS   Yoshioka S, Fujii W, Ogawa T, Sugiura K, Naito K
  TITLE     Development of a mono-promoter-driven CRISPR/Cas9 system in 
            mammalian cells.
  JOURNAL   Sci Rep. 2015 Dec 16;5:18341. doi: 10.1038/srep18341.
  PUBMED    26669567
REFERENCE   2  (bases 1 to 584)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2015-12-16"; volume: "5:18341. doi"; pages: " 
            10.1038/srep18341"
COMMENT     Sequence Label: pMCS-rybozyme-IRES-CAS9
FEATURES             Location/Qualifiers
     source          1..584
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        15..90
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     ncRNA           95..162
                     /product="hepatitis delta virus self-cleaving ribozyme 
                     (Ferre-D'Amare and Scott, 2010)"
                     /label=HDV ribozyme
                     /ncRNA_class="ribozyme"
     primer_bind     complement(427..444)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
ORIGIN
        1 gggatcccgt ctctgtttta gagctagaaa tagcaagtta aaataaggct agtccgttat
       61 caacttgaaa aagtggcacc gagtcggtgc ttttggccgg catggtccca gcctcctcgc
      121 tggcgccggc tgggcaacat gcttcggcat ggcgaatggg acaatcacta gtgaattcac
      181 tcctcaggtg caggctgcct atcagaaggt ggtggctggt gtggccaatg ccctggctca
      241 caaataccac tgagatccgc ccctctccct cccccccccc taacgttact ggccgaagcc
      301 gcttggaata aggccggtgt gcgtttgtct atatgttatt ttccaccata ttgccgtctt
      361 ttggcaatgt gagggcccgg aaacctggcc ctgtcttctt gacgagcatt cctaggggtc
      421 tttcccctct cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc
      481 tggaagcttc ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag cggaaccccc
      541 cacctggcga caggtgcctc tgcggccaaa agccacgtgt ataa
//
