LOCUS       sequence-116872-         688 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Recombine floxed alleles in newborn neurons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 688)
  AUTHORS   Williams MR, DeSpenza T Jr, Li M, Gulledge AT, Luikart BW
  TITLE     Hyperactivity of newborn Pten knock-out neurons results from 
            increased excitatory synaptic drive.
  JOURNAL   J Neurosci. 2015 Jan 21;35(3):943-59. doi: 
            10.1523/JNEUROSCI.3144-14.2015.
  PUBMED    25609613
REFERENCE   2  (bases 1 to 688)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1523/JNEUROSCI.3144-14"; journalName: "J Neurosci"; date: 
            "2015-01-21- 21"; volume: "35"; issue: "3"; pages: "943-59"
COMMENT     Sequence Label: pRubiC-T2A-cre
FEATURES             Location/Qualifiers
     source          1..688
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             247..300
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 atgtaccccg atgacggcgc cctgaagggc gagatcaagc agaggctgaa gctgaaggac
       61 ggcggccact acgacgctga ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg
      121 cccggcgcct acaacgtcaa catcaagttg gacatcacct cccacaacga ggactacacc
      181 atcgtggaac agtacgaacg cgccgagggc cgccactcca ccggcggcat ggacgagctg
      241 tacaaggagg gcagaggaag tcttctaaca tgcggtgacg tggaggagaa tcccggccct
      301 agtacatcca atttactgac cgtacaccaa aatttgcctg cattaccggt cgatgcaacg
      361 agtgatgagg ttcgcaagaa cctgatggac atgttcaggg atcgccaggc gttttctgag
      421 catacctgga aaatgcttct gtccgtttgc cggtcgtggg cggcatggtg caagttgaat
      481 aaccggaaat gctttcccgc agaacctgaa gatgttcgcg attatcttct atatcttcag
      541 gcgcgcggtc tggcagtaaa aactatccag caacatttgg gccagctaaa catgcttcat
      601 cgtcggtccg ggctgccacg accaagtgac agcaatgctg tttcactggt tatgcggcgg
      661 atccgaaaag aaaacgttga tgccggtg
//
