LOCUS       sequence-116875-         884 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  High molecular weight glutenin gene promoter driving GFP expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 884)
  AUTHORS   Vickers CE, Xue G, Gresshoff PM
  TITLE     A novel cis-acting element, ESP, contributes to high-level 
            endosperm-specific expression in an oat globulin promoter.
  JOURNAL   Plant Mol Biol. 2006 Sep;62(1-2):195-214. Epub 2006 Aug 17.
  PUBMED    16915522
REFERENCE   2  (bases 1 to 884)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant Mol 
            Biol."; date: "2006-09"; volume: "62(1-2)"; pages: "195-214. Epub 
            2006 Aug 17"
COMMENT     Sequence Label: pHMWGFPR
FEATURES             Location/Qualifiers
     source          1..884
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(380..398)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(381..398)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(479..498)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     598..620
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(658..676)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        744..848
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 agctgtacaa gtaaagcggc cgggctgcga ggagtctggt ggcaactaag ccgtcatcgt
       61 catatatagc ctcgtttaat tgttcatctc tgattcgatg atgtctccca ccttgtttcg
      121 tgtgttccca gtttgttcat cgtcttttga ttttaccggc cgtgctctgc ttttgttttt
      181 gtttcacctg atctctctct gacttgatgt aagagtggta tctgctacga ctatatgttg
      241 ttgggtgagg catatgtgaa tgaaatctat gaaagctccg gctatatata tttatacaaa
      301 gggtatgaga tggatgtgaa tcctagagca tatgtgtcca acaatcaatt cgtcgagggg
      361 gagcttcagc tgctcgagtt ctatagtgtc acctaaatcg tatgtgtatg atacataagg
      421 ttatgtatta attgtagccg cgttctaacg acaatatgtc catatggtgc actctcagta
      481 caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg
      541 cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg
      601 ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc
      661 tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag
      721 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
      781 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
      841 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccc
//
