LOCUS       sequence-116882-         729 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Express GFP in Newborn neurons.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 729)
  AUTHORS   Luikart BW, Bensen AL, Washburn EK, Perederiy JV, Su KG, Li Y, 
            Kernie SG, Parada LF, Westbrook GL
  TITLE     miR-132 mediates the integration of newborn neurons into the adult 
            dentate gyrus.
  JOURNAL   PLoS One. 2011;6(5):e19077. doi: 10.1371/journal.pone.0019077. Epub 
            2011 May 17.
  PUBMED    21611182
REFERENCE   2  (bases 1 to 729)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2011"; volume: "6(5):e19077. doi"; pages: " 
            10.1371/journal.pone.0019077. Epub 2011 May 17"
COMMENT     Sequence Label: pRubi-G
FEATURES             Location/Qualifiers
     source          1..729
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    52..640
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(105..125)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(523..534)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(643..659)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(644..660)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
ORIGIN
        1 tgtacaagta aagcggccgc gactctagaa ttcgatatca agcttatcga taatcaacct
       61 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg
      121 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc
      181 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt
      241 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc
      301 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg
      361 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact
      421 gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt
      481 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg
      541 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc
      601 cctcagacga gtcggatctc cctttgggcc gcctccccgc atcgataccg tcgacctcga
      661 gatatcagtg gtccaggctc tagttttgac tcaacaatat caccagctga agcctataga
      721 gtacgagcc
//
