LOCUS       sequence-116885-         865 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  encodes a non-targeting control shRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 865)
  AUTHORS   Deschenes-Simard X, Gaumont-Leclerc MF, Bourdeau V, Lessard F, 
            Moiseeva O, Forest V, Igelmann S, Mallette FA, Saba-El-Leil MK, 
            Meloche S, Saad F, Mes-Masson AM, Ferbeyre G
  TITLE     Tumor suppressor activity of the ERK/MAPK pathway by promoting 
            selective protein degradation.
  JOURNAL   Genes Dev. 2013 Apr 15;27(8):900-15. doi: 10.1101/gad.203984.112. 
            Epub 2013 Apr 18.
  PUBMED    23599344
REFERENCE   2  (bases 1 to 865)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1101/gad.203984.112"; journalName: "Genes Dev"; date: 
            "2013-04-15- 15"; volume: "27"; issue: "8"; pages: "900-15"
COMMENT     Sequence Label: shCTL-mlpx-puro
FEATURES             Location/Qualifiers
     source          1..865
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     ncRNA           14..134
                     /label=5' miR-30a
                     /note="sequence upstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           159..173
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           198..324
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     promoter        338..837
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(373..396)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     770..789
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
ORIGIN
        1 acagggtaat tgtttgaatg aggcttcagt actttacaga atcgttgcct gcacatcttg
       61 gaaacacttg ctgggattac ttcttcaggt taacccaaca gaaggctcga gaaggtatat
      121 gctgttgaca gtgagcgcgc gaagtctgta ctcttgtagt gaagccacag atgtacaaga
      181 gtacagactt cgcgtgccta ctgcctcgga attcaagggg ctactttagg agcaattatc
      241 ttgtttacta aaactgaata ccttgctatc tctttgatac atttttacaa agctgaatta
      301 aaatggtata aattaaatca cttttttcaa ttctaccggg taggggaggc gcttttccca
      361 aggcagtctg gagcatgcgc tttagcagcc ccgctgggca cttggcgcta cacaagtggc
      421 ctctggcctc gcacacattc cacatccacc ggtaggcgcc aaccggctcc gttctttggt
      481 ggccccttcg cgccaccttc tactcctccc ctagtcagga agttcccccc cgccccgcag
      541 ctcgcgtcgt gcaggacgtg acaaatggaa gtagcacgtc tcactagtct cgtgcagatg
      601 gacagcaccg ctgagcaatg gaagcgggta ggcctttggg gcagcggcca atagcagctt
      661 tgctccttcg ctttctgggc tcagaggctg ggaaggggtg ggtccggggg cgggctcagg
      721 ggcgggctca ggggcggggc gggcgcccga aggtcctccg gaggcccggc attctgcacg
      781 cttcaaaagc gcacgtctgc cgcgctgttc tcctcttcct catctccggg cctttcgacc
      841 tgcagcccaa gcttaccatg accga
//
