LOCUS       sequence-116890-        1072 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Theophylline Riboswitch E with luciferase gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1072)
  AUTHORS   Mishler DM, Gallivan JP
  TITLE     A family of synthetic riboswitches adopts a kinetic trapping 
            mechanism.
  JOURNAL   Nucleic Acids Res. 2014 Jun;42(10):6753-61. doi: 10.1093/nar/gku262.
            Epub 2014 Apr 29.
  PUBMED    24782524
REFERENCE   2  (bases 1 to 1072)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/nar/gku262"; journalName: "Nucleic Acids Res"; date: 
            "2014-06"; volume: "42"; issue: "10"; pages: "6753-61"
COMMENT     Sequence Label: pTac-E-luci
FEATURES             Location/Qualifiers
     source          1..1072
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             87..95
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     primer_bind     complement(171..189)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
ORIGIN
        1 ttaatcatcg gctcgtataa tgtgtgggac tcactatagg taccggtgat accagcatcg
       61 tcttgatgcc cttggcagca ccctgctaag gaggtaacaa caagatggaa gacgccaaaa
      121 acataaagaa aggcccggcg ccattctatc ctctagagga tggaaccgct ggagagcaac
      181 tgcataaggc tatgaagaga tacgccctgg ttcctggaac aattgctttt acagatgcac
      241 atatcgaggt gaacatcacg tacgcggaat acttcgaaat gtccgttcgg ttggcagaag
      301 ctatgaaacg atatgggctg aatacaaatc acagaatcgt cgtatgcagt gaaaactctc
      361 ttcaattctt tatgccggtg ttgggcgcgt tatttatcgg agttgcagtt gcgcccgcga
      421 acgacattta taatgaacgt gaattgctca acagtatgaa catttcgcag cctaccgtag
      481 tgtttgtttc caaaaagggg ttgcaaaaaa ttttgaacgt gcaaaaaaaa ttaccaataa
      541 tccagaaaat tattatcatg gattctaaaa cggattacca gggatttcag tcgatgtaca
      601 cgttcgtcac atctcatcta cctcccggtt ttaatgaata cgattttgta ccagagtcct
      661 ttgatcgtga caaaacaatt gcactgataa tgaattcctc tggatctact gggttaccta
      721 agggtgtggc ccttccgcat agaactgcct gcgtcagatt ctcgcatgcc agagatccta
      781 tttttggcaa tcaaatcatt ccggatactg cgattttaag tgttgttcca ttccatcacg
      841 gtttttggaa tgtttactac actcggatat ttgatatgtg gatttcgagt cgtcttaatg
      901 tatagatttg aagaagagct gtttttacga tccccttcag gattacaaat tcaagtgcgt
      961 tgctagtaca acccctattt tcattcttcg ccaaagcact ctgattgaca aatacgattt
     1021 atctaattta cacaaaattg cttctggggg cgcacctctt tcgaaagaag tc
//
