LOCUS       sequence-116891-         808 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses RFP-tagged RNAseH1 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 808)
  TITLE     RNAseH1 plasmids (unpublished)
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 808)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pFRT-TODestRFP_RNAseH1
FEATURES             Location/Qualifiers
     source          1..808
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    11..29
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    32..50
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     complement(421..439)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     689..708
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 agcagagctc tccctatcag tgatagagat ctccctatca gtgatagaga tcgtcgacga
       61 gctcgtttta gtgaaccgtc agatcgcctg gagacgccat ccacgctgtt ttgacctcca
      121 tagaagacac cgggaccgat ccagcctccg gactctagcg tttaaactta agcttggtac
      181 cgagctcgga tccatggtga gcaagggcga ggggtaccat ggtgagcaag ggggtaccat
      241 ggtgagcaag ggcgggggta ccaggtgagc aagggcgagg gggtaccatg gtgagcaagg
      301 gcgaggaggt catcaaagag ttcatgcgct tcaaggtgcg catggagggc tccgtgaacg
      361 gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc acccagaccg
      421 ccaagctgaa ggtgaccaag ggcggccccc tgcccttcgc ctgggacatc ctgtcccccc
      481 agttcatgta cggctccaag gcgtacgtga agcaccccgc cgacatcccc gattacaaga
      541 agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag gacggcggtc
      601 tggtgaccgt gacccaggac tcctccctgc aggacggcac gctgatctac aaggtgaaga
      661 tgcgcggcac caacttcccc cccgacggcc ccgtaatgca gaagaagacc atgggctggg
      721 aggcctccac cgagcgcctg tacccccgcg acggcgtgct gaagggcgag atccaccagg
      781 ccctgaagct gaaggacggc ggccacta
//
