LOCUS       sequence-116893-         310 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses CAS9-eGFP fusion protein in mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 310)
  AUTHORS   Fujii W, Kawasaki K, Sugiura K, Naito K
  TITLE     Efficient generation of large-scale genome-modified mice using gRNA 
            and CAS9 endonuclease.
  JOURNAL   Nucleic Acids Res. 2013 Aug 30.
  PUBMED    23997119
REFERENCE   2  (bases 1 to 310)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2013 Aug 30."
COMMENT     Sequence Label: pCAG-T3-hCASeGFP-pA
FEATURES             Location/Qualifiers
     source          1..310
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             25..48
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             58..78
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 tctagcccgg gcggatccac catggattac aaggatgacg acgataagat catggcccca
       61 aagaagaagc ggaaggtcgg tatccacgga gtcccagcag ccgacaagaa gtactccatt
      121 gggctcgata tcggcacaaa cagcgtcggc tgggccgtca ttacggacga gtacaaggtg
      181 ccgagcaaaa aattcaaagt tctgggcaat accgatcgcc acagcataaa gaagaacctc
      241 attggcgccc tcctgttcga ctccggggag acggccgaag ccacgcggct caaaagaaca
      301 gcacggcgca
//
