LOCUS       sequence-116896-         595 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses RFP-tagged HuD in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 595)
  TITLE     Identification of the transcriptome-wide mRNA targets of the 
            neuronal ELAVL proteins (unpublished)
REFERENCE   2  (bases 1 to 595)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pFRT-TODestRFP_HuD
FEATURES             Location/Qualifiers
     source          1..595
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    31..49
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     59..83
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     complement(419..437)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 agcagagctc tcctatcagt gatagagatc tccctatcag tgatagagat cgtcgacgag
       61 ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt gacctccata
      121 gaagacaccg ggaccgatcc agcctccgga ctctagcgtt taaacttaag cttggtaccg
      181 agctcggatc catggtgagc aagggcgagg ggtaccatgg tgagcaaggg ggtaccatgg
      241 tgagcaaggg cgggggtacc aggtgagcaa gggcgagggg gtaccatggt gagcaagggc
      301 gaggaggtca tcaaagagtt catgcgcttc aaggtgcgca tggagggctc cgtgaacggc
      361 cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac ccagaccgcc
      421 aagctgaagg tgaccaaggg cggccccctg cccttcgcct gggacatcct gtccccccag
      481 ttcatgtacg gctccaaggc gtacgtgaag caccccgccg acatccccga ttacaagaag
      541 ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga cggcg
//
