LOCUS       sequence-116898-         896 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  No-Aptamer Control for TMR Riboswitch S02 with luciferase gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 896)
  TITLE     Biophysical Modeling for Riboswitches
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 896)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pTMR-S02-NAC
FEATURES             Location/Qualifiers
     source          1..896
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             59..67
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     primer_bind     complement(138..156)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
ORIGIN
        1 attcccgtac cggatcgcga ctggcgagag ccaggtaacg aatcgatcca agattcgtta
       61 aggaggtcca tatggaagac gccaaaaaca taaagaaagg cccggcgcca ttctatcctc
      121 tagaggatgg aaccgctgga gagcaactgc ataaggctat gaagagatac gccctggttc
      181 ctggaacaat tgcttttaca gatgcacata tcgaggtgaa catcacgtac gcggaatact
      241 tcgaaatgtc cgttcggttg gcagaagcta tgaaacgata tgggctgaat acaaatcaca
      301 gaatcgtcgt atgcagtgaa aactctcttc aattctttat gccggtgttg ggcgcgttat
      361 ttatcggagt tgcagttgcg cccgcgaacg acatttataa tgaacgtgaa ttgctcaaca
      421 gtatgaacat ttcgcagcct accgtagtgt ttgtttccaa aaaggggttg caaaaaattt
      481 tgaacgtgca aaaaaaatta ccaataatcc agaaaattat tatcatggat tctaaaacgg
      541 attaccaggg atttcagtcg atgtacacgt tcgtcacatc tcatctacct cccggtttta
      601 atgaatacga ttttgtacca gagtcctttg atcgtgacaa aacaattgca ctgataatga
      661 attcctctgg atctactggg ttacctaagg gtgtggccct tccgcataga actgcctgcg
      721 tcagattctc gcatgccaga gatcctattt ttggcaatca aatcattccg gatactgcga
      781 ttttaagtgt tgttccattc catcacggtt ttggaatgtt tactacactc ggatatttga
      841 tatgtgggat ttcgagtcgt ctttatgtat agaattttga aggaagagct ggtttt
//
