LOCUS       sequence-116901-         870 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  plasmid for bacterial overexpression of RBPMS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 870)
  AUTHORS   Farazi TA, Leonhardt CS, Mukherjee N, Mihailovic A, Li S, Max KE, 
            Meyer C, Yamaji M, Cekan P, Jacobs NC, Gerstberger S, Bognanni C, 
            Larsson E, Ohler U, Tuschl T
  TITLE     Identification of the RNA recognition element of the RBPMS family of
            RNA-binding proteins and their transcriptome-wide mRNA targets.
  JOURNAL   RNA. 2014 Jul;20(7):1090-102. doi: 10.1261/rna.045005.114. Epub 2014
            May 23.
  PUBMED    24860013
REFERENCE   2  (bases 1 to 870)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1261/rna.045005.114"; journalName: "RNA"; date: "2014-07"; 
            volume: "20"; issue: "7"; pages: "1090-102"
COMMENT     Sequence Label: pet23a_RBPMS_1_111
FEATURES             Location/Qualifiers
     source          1..870
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             15..47
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             426..443
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(496..514)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      510..557
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     primer_bind     complement(681..700)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 aaggagatat acatatggct agcatgactg gtggacagca aatgggtcgc ggatccgaat
       61 tcgagctccg tcgacccatg aacaacggcg gcaaagccga gaaggagaac accccgagcg
      121 aggccaacct tcaggaggag gaggtccgga ccctatttgt cagtggcctt cctctggata
      181 tcaaacctcg ggagctctat ctgcttttca gaccatttaa gggctatgag ggttctctta
      241 taaagctcac atctaaacag cctgtaggtt ttgtcagttt tgacagtcgc tcagaagcag
      301 aggctgcaaa gaatgctttg aatggcatcc gcttcgatcc tgaaattccg caaacactac
      361 gactagagtt tgctaaggca aacacgaaga tggccaagaa caaactcgta gcggccgcac
      421 tcgagcacca ccaccaccac cactgagatc cggctgctaa caaagcccga aaggaagctg
      481 agttggctgc tgccaccgct gagcaataac tagcataacc ccttggggcc tctaaacggg
      541 tcttgagggg ttttttgctg aaaggaggaa ctatatccgg attggcgaat gggacgcgcc
      601 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
      661 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
      721 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
      781 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc
      841 ctgatagacg gtttttcgcc ctttgacgtt
//
