LOCUS       sequence-116905-         883 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of PIP2 binding protein in sea urchin embryos.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 883)
  AUTHORS   Campanale JP, Gokirmak T, Espinoza JA, Oulhen N, Wessel GM, Hamdoun 
            A
  TITLE     Migration of sea urchin primordial germ cells.
  JOURNAL   Dev Dyn. 2014 Jul;243(7):917-27. doi: 10.1002/dvdy.24133. Epub 2014 
            Apr 30.
  PUBMED    24677545
REFERENCE   2  (bases 1 to 883)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1002/dvdy.24133"; journalName: "Dev Dyn"; date: "2014-07"; 
            volume: "243"; issue: "7"; pages: "917-27"
COMMENT     Sequence Label: NmCitrine-PHdomain
FEATURES             Location/Qualifiers
     source          1..883
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(592..843)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(634..655)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 tcactatagt tctagaggct cgagttactg gatgttgagc tccttcagga agttctgcag
       61 ctccttgaag ctcatcttgt tgtccttgtt tttgtcagct tttcgcaagc aggagtgaat
      121 ccagtgctgt agcttctgac gctggtccat ggagcctgag tggtggatga tcttgtgcag
      181 ccccagcacc cagtgctggg catcagctgg cgatggggcg atgaggtcta gtgtattgcg
      241 ctggtccttg aagacaatgg agaagcagcg gtcctcgggc acatcacggg cgaacttctc
      301 cagaccctcc gtgcggtgcc ccattcgcac ctcctgaatg tcctcgatgg agaacagctg
      361 ggactccggg gtccgcatga ccttgcggga ctcctgccag atggtcttgc agtcctcctg
      421 caacttgtag aagcgctctc tcctccatga gctggacttc accttcagga gctggctgcc
      481 cttcagcagc gcctgtagat cctcatcatc ctgtaggccg tgcagggtca ggaagtcccg
      541 gcccgagtcc attggccggc ctttaattaa tggcgcgccg gctccgaatt ccttgtacag
      601 ctcgtccatg ccgagagtga tcccggcggc ggtcacgaac tccagcagga ccatgtgatc
      661 gcgcttctcg ttggggtctt tgctcagggc ggactggtag ctcaggtagt ggttgtcggg
      721 cagcagcacg gggccgtcgc cgatgggggt gttctgctgg tagtggtcgg cgagctgcac
      781 gctgccgtcc tcgatgttgt ggcggatctt gaagttcacc ttgatgccgt tcttctgctt
      841 gtcggccatg atatagacgt tgtggctgtt gtagttgtac tcc
//
