LOCUS       sequence-116907-         870 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of PIP2 binding protein in sea urchin embryos.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 870)
  AUTHORS   Campanale JP, Gokirmak T, Espinoza JA, Oulhen N, Wessel GM, Hamdoun 
            A
  TITLE     Migration of sea urchin primordial germ cells.
  JOURNAL   Dev Dyn. 2014 Jul;243(7):917-27. doi: 10.1002/dvdy.24133. Epub 2014 
            Apr 30.
  PUBMED    24677545
REFERENCE   2  (bases 1 to 870)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1002/dvdy.24133"; journalName: "Dev Dyn"; date: "2014-07"; 
            volume: "243"; issue: "7"; pages: "917-27"
COMMENT     Sequence Label: Ncerulean-PHdomain
FEATURES             Location/Qualifiers
     source          1..870
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        33..51
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     33..50
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             join(98..100,101..103,104..814)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAISDNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(143..164)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(404..423)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     751..772
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ccattctgcc tggggacgtc ggagcaagct tgatttaggt gacactatag aatacaagct
       61 acttgttctt tttgcaggat ccactagtca tcgattgatg gtgagcaagg gcgaggagct
      121 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg gccacaagtt
      181 cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat
      241 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc tgacctgggg
      301 cgtgcagtgc ttcgcccgct accccgacca catgaagcag cacgacttct tcaagtccgc
      361 catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa
      421 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg
      481 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca acgccatcag
      541 cgacaacgtc tatatcaccg ccgacaagca gaagaacggc atcaaggcca acttcaagat
      601 ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc
      661 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccaagct
      721 gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc
      781 cgggatcact ctcggcatgg acgagctgta caaggaattc ggagccggcg cgccattaat
      841 taaaggccgg ccaatggact cgggccggga
//
