LOCUS       sequence-116910-         844 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MYC expression vector with RBPMS2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 844)
  AUTHORS   Teplova M, Farazi TA, Tuschl T, Patel DJ
  TITLE     Structural basis underlying CAC RNA recognition by the RRM domain of
            dimeric RNA-binding protein RBPMS.
  JOURNAL   Q Rev Biophys. 2015 Sep 8:1-11.
  PUBMED    26347403
REFERENCE   2  (bases 1 to 844)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Q Rev 
            Biophys. 2015 Sep 8:1-11."
COMMENT     Sequence Label: pFRT-TODestMYC_RBPMS2
FEATURES             Location/Qualifiers
     source          1..844
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    11..29
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    32..50
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     60..84
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             184..213
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     protein_bind    220..244
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 agcagagctc tccctatcag tgatagagat ctccctatca gtgatagaga tcgtcgacga
       61 gctcgtttag tgaaccgtca gatcgcctgg agacgccatc cacgctgttt tgacctccat
      121 agaagacacc gggaccgatc cagcctccgg actctagcgt ttaaacttaa gcttggtacc
      181 atggagcaga aactcatctc tgaagaggat ctgaagctta caagtttgta caaaaaagca
      241 ggcttcatga gcaacctgaa gccggacggc gagcacggcg gcagcaccgg caccggctcc
      301 ggcgcgggct ccggcggcgc cctggaggag gaggtccgga cactgtttgt cagcggcctc
      361 cctgtggaca ttaaacccag agaactctac ttgctcttcc ggccgttcaa ggggtatgaa
      421 gggtccctga tcaagctcac tgcaagacag cctgttggtt ttgtgatctt tgacagccgt
      481 gcaggagcag aagcggccaa gaatgcgctg aacggtattc gctttgatcc cgaaaatcca
      541 cagactctga ggctagagtt tgccaaagcc aacaccaaga tggccaagag caagctaatg
      601 gcaactccaa atcccagcaa cgtgcacccc gccctaggag cacacttcat cgcacgggac
      661 ccctatgacc tgatgggggc tgctctgatc cctgcatccc cagaggcctg ggccccctac
      721 cctttgtaca ccacagagct gaccccagcc atctcccatg ctgcgttcac ctacccaact
      781 gccactgccg ctgccgccgc cctccacgct caggtgcgct ggtacccttc ctctgacacc
      841 accc
//
