LOCUS       sequence-116912-         821 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  plasmid for bacterial overexpression of RBPMS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 821)
  AUTHORS   Farazi TA, Leonhardt CS, Mukherjee N, Mihailovic A, Li S, Max KE, 
            Meyer C, Yamaji M, Cekan P, Jacobs NC, Gerstberger S, Bognanni C, 
            Larsson E, Ohler U, Tuschl T
  TITLE     Identification of the RNA recognition element of the RBPMS family of
            RNA-binding proteins and their transcriptome-wide mRNA targets.
  JOURNAL   RNA. 2014 Jul;20(7):1090-102. doi: 10.1261/rna.045005.114. Epub 2014
            May 23.
  PUBMED    24860013
REFERENCE   2  (bases 1 to 821)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1261/rna.045005.114"; journalName: "RNA"; date: "2014-07"; 
            volume: "20"; issue: "7"; pages: "1090-102"
COMMENT     Sequence Label: pet23a_RBPMS_20_196
FEATURES             Location/Qualifiers
     source          1..821
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             16..48
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             628..645
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(698..716)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      712..759
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 gaaggagata tacatatggc tagcatgact ggtggacagc aaatgggtcg cggatccgaa
       61 ttcgagctcc gtcgacccat ggaggaggag gtccggaccc tatttgtcag tggccttcct
      121 ctggatatca aacctcggga gctctatctg cttttcagac catttaaggg ctatgagggt
      181 tctcttataa agctcacatc taaacagcct gtaggttttg tcagttttga cagtcgctca
      241 gaagcagagg ctgcaaagaa tgctttgaat ggcatccgct tcgatcctga aattccgcaa
      301 acactacgac tagagtttgc taaggcaaac acgaagatgg ccaagaacaa actcgtaggg
      361 actccaaacc ccagtactcc tctgcccaac actgtacctc agttcattgc cagagagcca
      421 tatgagctca cagtgcctgc actttacccc agtagccctg aagtgtgggc cccgtaccct
      481 ctgtacccag cggagttagc gcctgctcta cctcctcctg ctttcaccta tcccgcttca
      541 ctgcatgccc agatgcgctg gctccctccc tccgaggcta cttctcaggg ctggaagtcc
      601 cgtcagttct gcgcggccgc actcgagcac caccaccacc accactgaga tccggctgct
      661 aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa
      721 ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc
      781 ggattggcga atgggacgcg ccctgtagcg gcgcattaag c
//
