LOCUS       sequence-116923-         966 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  TMR Riboswitch S02 with luciferase gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 966)
  TITLE     Biophysical Modeling for Riboswitches
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 966)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pTMR-S02
FEATURES             Location/Qualifiers
     source          1..966
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             84..92
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     primer_bind     complement(163..181)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
ORIGIN
        1 atcatcggct cgtataatgt gtgggattcc cgtaccggat cgcgactggc gagagccagg
       61 taacgaatcg atccaagatt cgttaaggag gtccatatgg aagacgccaa aaacataaag
      121 aaaggcccgg cgccattcta tcctctagag gatggaaccg ctggagagca actgcataag
      181 gctatgaaga gatacgccct ggttcctgga acaattgctt ttacagatgc acatatcgag
      241 gtgaacatca cgtacgcgga atacttcgaa atgtccgttc ggttggcaga agctatgaaa
      301 cgatatgggc tgaatacaaa tcacagaatc gtcgtatgca gtgaaaactc tcttcaattc
      361 tttatgccgg tgttgggcgc gttatttatc ggagttgcag ttgcgcccgc gaacgacatt
      421 tataatgaac gtgaattgct caacagtatg aacatttcgc agcctaccgt agtgtttgtt
      481 tccaaaaagg ggttgcaaaa aattttgaac gtgcaaaaaa aattaccaat aatccagaaa
      541 attattatca tggattctaa aacggattac cagggatttc agtcgatgta cacgttcgtc
      601 acatctcatc tacctcccgg ttttaatgaa tacgattttg taccagagtc ctttgatcgt
      661 gacaaaacaa ttgcactgat aatgaattcc tctggatcta ctgggttacc taagggtgtg
      721 gcccttccgc atagaactgc ctgcgtcaga ttctcgcatg ccagagatcc tatttttggc
      781 aatcaaatca ttccggatac tgcgattttt aagtgttgtt ccatttccat cacggttttg
      841 gaatgtttta ctacactcgg atatttgata tgtgggattt cgagtcgtct taatgtatag
      901 aattgaagaa gagctgtttt tacgatcccc ttccaggatt acaaaatttc aaaagtgcgt
      961 tggcta
//
