LOCUS       sequence-116927-         983 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Cre recombinase with a 25 nucleotide extracellular vesicle
            targeting sequence under the UBC promotor and CFP under the CMV 
            promotor..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 983)
  AUTHORS   Zomer A, Maynard C, Verweij FJ, Kamermans A, Schafer R, Beerling E, 
            Schiffelers RM, de Wit E, Berenguer J, Ellenbroek SI, Wurdinger T, 
            Pegtel DM, van Rheenen J
  TITLE     In Vivo imaging reveals extracellular vesicle-mediated phenocopying 
            of metastatic behavior.
  JOURNAL   Cell. 2015 May 21;161(5):1046-57. doi: 10.1016/j.cell.2015.04.042.
  PUBMED    26000481
REFERENCE   2  (bases 1 to 983)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2015.04"; journalName: "Cell"; date: "2015-05-21- 
            21"; volume: "161"; issue: "5"; pages: "1046-57"
COMMENT     Sequence Label: pcDNA3.1-CMV-CFP;UBC-Cre25nt
FEATURES             Location/Qualifiers
     source          1..983
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     61..80
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        61..79
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      102..111
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(108..110,111..113,114..827)
                     /codon_start=1
                     /product="enhanced CFP"
                     /label=ECFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(153..174)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(414..433)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     761..782
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    837..948
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 ctatataagc agagctctct ggctaactag agaacccact gcttactggc ttatcgaaat
       61 taatacgact cactataggg agacccaagc tggctagctt tgccaccatg gtgagcaagg
      121 gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg
      181 gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc
      241 tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc
      301 tgacctgggg cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct
      361 tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg
      421 gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg
      481 agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca
      541 actacatcag ccacaacgtc tatatcaccg ccgacaagca gaagaacggc atcaaggcca
      601 acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc
      661 agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc
      721 agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg
      781 tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagtaagga tcccgactgt
      841 gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga
      901 aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag
      961 taggtgtcat tctattctgg ggg
//
