LOCUS       sequence-116931-        1008 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Theophylline Riboswitch B with luciferase gene.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1008)
  AUTHORS   Mishler DM, Gallivan JP
  TITLE     A family of synthetic riboswitches adopts a kinetic trapping 
            mechanism.
  JOURNAL   Nucleic Acids Res. 2014 Jun;42(10):6753-61. doi: 10.1093/nar/gku262.
            Epub 2014 Apr 29.
  PUBMED    24782524
REFERENCE   2  (bases 1 to 1008)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1093/nar/gku262"; journalName: "Nucleic Acids Res"; date: 
            "2014-06"; volume: "42"; issue: "10"; pages: "6753-61"
COMMENT     Sequence Label: pTac-B-luci
FEATURES             Location/Qualifiers
     source          1..1008
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(179..197)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
ORIGIN
        1 ttgacattaa tcatcggctc gtataatgtg tgggactcac tataggtacc ggtgatacca
       61 gcatcgtctt gatgcccttg gcagcacccg ctgcgcaggg ggtatcaaca agatggaaga
      121 cgccaaaaac ataaagaaag gcccggcgcc attctatcct ctagaggatg gaaccgctgg
      181 agagcaactg cataaggcta tgaagagata cgccctggtt cctggaacaa ttgcttttac
      241 agatgcacat atcgaggtga acatcacgta cgcggaatac ttcgaaatgt ccgttcggtt
      301 ggcagaagct atgaaacgat atgggctgaa tacaaatcac agaatcgtcg tatgcagtga
      361 aaactctctt caattcttta tgccggtgtt gggcgcgtta tttatcggag ttgcagttgc
      421 gcccgcgaac gacatttata atgaacgtga attgctcaac agtatgaaca tttcgcagcc
      481 taccgtagtg tttgtttcca aaaaggggtt gcaaaaaatt ttgaacgtgc aaaaaaaatt
      541 accaataatc cagaaaatta ttatcatgga ttctaaaacg gattaccagg gatttcagtc
      601 gatgtacacg ttcgtcacat ctcatctacc tcccggtttt aatgaatacg attttgtacc
      661 agagtccttt gatcgtgaca aaacaattgc actgataatg aattcctctg gatctactgg
      721 gttacctaag ggtgtggccc ttccgcatag aactgcctgc gtcagattct cgcatgccag
      781 agatcctatt tttggcaatc aaatcattcc ggatactgcg attttaagtg ttgttccatt
      841 ccatcacggt tttggaatgt tttactacac tcggatattt gatatgtgga tttcgagtcg
      901 tcttaatgta tagatttgga agaaagagct ggtttttacg atcccttcag gattacaaaa
      961 ttcaaaagtg cgttgcctag tacccaaccc ctattttcat tcttcgcc
//
