LOCUS       sequence-116933-         731 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Carries CA repeats of 50-100 nucleotides in length, an RBPMS target.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 731)
  TITLE     RNA-binding protein 3' UTR target plasmids (unpublished)
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 731)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pDONR221_50_100_CA_repeat
FEATURES             Location/Qualifiers
     source          1..731
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(229..248)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(230..248)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(255..272)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(255..271)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(264..286)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             409..711
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="QFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKV
                     SRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     590..609
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 ctcaagctat gcatcaagct tggtaccgag ctcggatcca ctagtaacgg ccgccagtgt
       61 gctggaattc aggcacacac acacacacgg caccacacac acacacacac acggcaccac
      121 acacacacac acacacggca ccacacacac acacacacac ggcctgaatt ctgcagatat
      181 ccatcacact ggcggccgct cgagcatgca tctagagggc ccaattcgcc ctatagtgag
      241 tcgtattaca attcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt
      301 acccaactta atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag
      361 gcccgcaccg atcgcccttc ccaacagttg cgcagcctat acgtacggca gtttaaggtt
      421 tacacctata aaagagagag ccgttatcgt ctgtttgtgg atgtacagag tgatattatt
      481 gacacgccgg ggcgacggat ggtgatcccc ctggccagtg cacgtctgct gtcagataaa
      541 gtctcccgtg aactttaccc ggtggtgcat atcggggatg aaagctggcg catgatgacc
      601 accgatatgg ccagtgtgcc ggtctccgtt atcggggaag aagtggctga tctcagccac
      661 cgcgaaaatg acatcaaaaa cgccattaac ctgatgttct ggggaatata aatgtcaggc
      721 atgagattat c
//
