LOCUS       sequence-116936-        1014 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses Cre recombinase with a 25 nucleotide extracellular vesicle
            targeting sequence under the UBC promotor and CFP under the CMV 
            promotor..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1014)
  AUTHORS   Zomer A, Maynard C, Verweij FJ, Kamermans A, Schafer R, Beerling E, 
            Schiffelers RM, de Wit E, Berenguer J, Ellenbroek SI, Wurdinger T, 
            Pegtel DM, van Rheenen J
  TITLE     In Vivo imaging reveals extracellular vesicle-mediated phenocopying 
            of metastatic behavior.
  JOURNAL   Cell. 2015 May 21;161(5):1046-57. doi: 10.1016/j.cell.2015.04.042.
  PUBMED    26000481
REFERENCE   2  (bases 1 to 1014)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2015.04"; journalName: "Cell"; date: "2015-05-21- 
            21"; volume: "161"; issue: "5"; pages: "1046-57"
COMMENT     Sequence Label: pcDNA3.1-CMV-CFP;UBC-Cre25nt
FEATURES             Location/Qualifiers
     source          1..1014
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    119..343
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     332..349
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
ORIGIN
        1 ctggcaagtg tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg
       61 ctacagggcg cgtggggata ccccctagag ccccagctgg ttctttccgc ctcagaagcc
      121 atagagccca ccgcatcccc agcatgcctg ctattgtctt cccaatcctc ccccttgctg
      181 tcctgcccca ccccaccccc cagaatagaa tgacacctac tcagacaatg cgatgcaatt
      241 tcctcatttt attaggaaag gacagtggga gtggcacctt ccagggtcaa ggaaggcacg
      301 ggggaggggc aaacaacaga tggctggcaa ctagaaggca cagtcgaggc tgatcagcgg
      361 gtttaaacgg gccctctaga ggattcacag gcggagtcca ggcggcaggg tggattccta
      421 atcgccatct tccagcaggc gcaccattgc ccctgtttca ctatccaggt tacggatata
      481 gttcatgaca atatttacat tggtccagcc accagcttgc atgatctccg gtattgaaac
      541 tccagcgcgg gccatatctc gcgcggctcc gacacgggca ctgtgtccag accaggccag
      601 gtatctctga ccagagtcat ccttagcgcc gtaaatcaat cgatgagttg cttcaaaaat
      661 cccttccagg gcgcgagttg atagctggct ggtggcagat ggcgcggcaa caccattttt
      721 tctgacccgg caaaacaggt agttattcgg atcatcagct acaccagaga cggaaatcca
      781 tcgctcgacc agtttagtta cccccaggct aagtgccttc tctacacctg cggtgctaac
      841 cagcgttttc gttctgccaa tatggattaa cattctccca ccgtcagtac gtgagatatc
      901 tttaaccctg atcctggcaa tttcggctat acgtaacagg gtgttataag caatccccag
      961 aaatgccaga ttacgtatat cctggcagcg atcgctattt tccatgagtg aacg
//
