LOCUS       sequence-116938-         780 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Carries CA repeats of 50 nucleotides in length, an RBPMS target.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 780)
  TITLE     RNA-binding protein 3' UTR target plasmids (unpublished)
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 780)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pDONR221_50_CA_repeat
FEATURES             Location/Qualifiers
     source          1..780
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(200..219)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(201..219)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(226..243)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(226..242)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(235..257)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     CDS             380..682
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="QFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKV
                     SRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     561..580
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 actcaagcta tgcatcaagc ttggtaccga gctcggatcc actagtaacg gccgccagtg
       61 tgctggaatt caggacacac ggcaccacac acacacacac acacggcacc acacacacac
      121 acacacacgg caccctgaat tctgcagata tccatcacac tggcggccgc tcgagcatgc
      181 atctagaggg cccaattcgc cctatagtga gtcgtattac aattcactgg ccgtcgtttt
      241 acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc
      301 ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt
      361 gcgcagccta tacgtacggc agtttaaggt ttacacctat aaaagagaga gccgttatcg
      421 tctgtttgtg gatgtacaga gtgatattat tgacacgccg gggcgacgga tggtgatccc
      481 cctggccagt gcacgtctgc tgtcagataa agtctcccgt gaactttacc cggtggtgca
      541 tatcggggat gaaagctggc gcatgatgac caccgatatg gccagtgtgc cggtctccgt
      601 tatcggggaa gaagtggctg atctcagcca ccgcgaaaat gacatcaaaa acgccattaa
      661 cctgatgttc tggggaatat aaatgtcagg catgagatta tcaaaaagga tcttcaccta
      721 gatccttttc acgtagaaag ccagtccgca gaaacggtgc tgaccccgga tgaatgtcag
//
