LOCUS       sequence-116941-         898 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of PIP2 binding protein in sea urchin embryos.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 898)
  AUTHORS   Campanale JP, Gokirmak T, Espinoza JA, Oulhen N, Wessel GM, Hamdoun 
            A
  TITLE     Migration of sea urchin primordial germ cells.
  JOURNAL   Dev Dyn. 2014 Jul;243(7):917-27. doi: 10.1002/dvdy.24133. Epub 2014 
            Apr 30.
  PUBMED    24677545
REFERENCE   2  (bases 1 to 898)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1002/dvdy.24133"; journalName: "Dev Dyn"; date: "2014-07"; 
            volume: "243"; issue: "7"; pages: "917-27"
COMMENT     Sequence Label: Ncerulean-PHdomain
FEATURES             Location/Qualifiers
     source          1..898
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(596..847)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSTQSKLSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(638..659)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 cgactcacta tagttctaga ggctcgagtt actggatgtt gagctccttc aggaagttct
       61 gcagctcctt gaagctcatc ttgttgtcct tgtttttgtc agcttttcgc aagcaggagt
      121 gaatccagtg ctgtagcttc tgacgctggt ccatggagcc tgagtggtgg atgatcttgt
      181 gcagccccag cacccagtgc tgggcatcag ctggcgatgg ggcgatgagg tctagtgtat
      241 tgcgctggtc cttgaagaca atggagaagc agcggtcctc gggcacatca cgggcgaact
      301 tctccagacc ctccgtgcgg tgccccattc gcacctcctg aatgtcctcg atggagaaca
      361 gctgggactc cggggtccgc atgaccttgc gggactcctg ccagatggtc ttgcagtcct
      421 cctgcaactt gtagaagcgc tctctcctcc atgagctgga cttcaccttc aggagctggc
      481 tgcccttcag cagcgcctgt agatcctcat catcctgtag gccgtgcagg gtcaggaagt
      541 cccggcccga gtccattggc cggcctttaa ttaatggcgc gccggctccg aattccttgt
      601 acagctcgtc catgccgaga gtgatcccgg cggcggtcac gaactccagc aggaccatgt
      661 gatcgcgctt ctcgttgggg tctttgctca gcttggactg ggtgctcagg tagtggttgt
      721 cgggcagcag cacggggccg tcgccgatgg gggtgttctg ctggtagtgg tcggcgagct
      781 gcacgctgcc gtcctcgatg ttgtggcgga tcttgaagtt ggccttgatg ccgttcttct
      841 gcttgtcggc ggtgatatag acgttgtcgc tgatggcgtt gtactccagc ttgtgccc
//
