LOCUS       sequence-116948-        1081 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral vector for pHRed expression in mammalian cells using the 
            hUbiC promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1081)
  AUTHORS   Tantama M, Martinez-Francois JR, Mongeon R, Yellen G
  TITLE     Imaging energy status in live cells with a fluorescent biosensor of 
            the intracellular ATP-to-ADP ratio.
  JOURNAL   Nat Commun. 2013;4:2550. doi: 10.1038/ncomms3550.
  PUBMED    24096541
REFERENCE   2  (bases 1 to 1081)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2013"; volume: "4:2550. doi"; pages: " 
            10.1038/ncomms3550"
COMMENT     Sequence Label: FUGW-pHRed
FEATURES             Location/Qualifiers
     source          1..1081
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(143..802,803..805,806..808))
                     /codon_start=1
                     /product="red fluorescent protein that serves as a 
                     ratiometric pH sensor (Tantama et al., 2011)"
                     /label=pHRed
                     /note="contains an A213S mutation relative to the parental 
                     mKeima protein"
                     /translation="MVSVIAKQMTYKVYMSGTVNGHYFEVEGDGKGKPYEGEQTVKLTV
                     TKGGPLPFAWDILSPQLQYGSIPFTKYPEDIPDYFKQSFPEGYTWERSMNFEDGAVCTV
                     SNDSSIQGNCFIYNVKISGENFPPNGPVMQKKTQGWEPSTERLFARDGMLIGNDYMALK
                     LEGGGHYLCEFKSTYKAKKPVRMPGRHEIDRKLDVTSHNRDYTSVEQCEISIARHSLLG
                     "
     primer_bind     complement(986..1005)
                     /label=hUBCpro-F
                     /note="Human Ubiquitin C (UbC) promoter, forward primer"
ORIGIN
        1 gtcaatcttt cacaaatttt gtaatccaga ggttgattat cgataagctt gatatcgaat
       61 tgttaacgga tccgctagct tcgaagaatt cgtcgactca gctagcacca ccagagccac
      121 caccgtcggc ggagttgcct gtccccagca gagagtggcg ggcgatagag atttcacact
      181 gctcaacgct ggtgtagtcg cggttgtgag aggtaacatc cagcttgcgg tcgatttcgt
      241 ggcggccagg catgcgaaca ggctttttag ccttgtaagt gctcttgaac tcgcacaggt
      301 agtgaccgcc gccttccagt ttcagggcca tataatcgtt accaatcagc ataccgtcgc
      361 gggcaaacag gcgttcggtg ctgggctccc agccttgggt tttcttctgc ataactgggc
      421 cgttaggtgg gaagttctcg ccggaaatct taacgttgta gatgaagcag ttaccctgga
      481 tggagctgtc gttggaaacg gtacaaactg cgccgtcctc gaagttcata gagcgctccc
      541 aggtgtagcc ctctgggaag ctctgcttaa agtagtcagg gatgtcctcg gggtatttgg
      601 tgaaagggat ggagccgtat tggagctgag gggacaggat atcccaggcg aaaggcagtg
      661 ggccgccttt ggtaacggtc agcttaacgg tctgctcgcc ctcgtaaggc ttgcccttgc
      721 cgtcaccttc aacctcgaag tagtggccgt taacggtgcc agacatataa actttatagg
      781 tcatttgctt tgcgataacg gaaaccatgg tggtctagag tcgacctgca gcccaagctt
      841 cgtctaacaa aaaagccaaa aacggccaga atttagcgga caatttacta gtctaacact
      901 gaaaattaca tattgaccca aatgattaca tttcaaaagg tgcctaaaaa acttcacaaa
      961 acacactcgc caaccccgag cgcatagttc aaaaccggag cttcagctac ttaagaagat
     1021 aggtacataa aaccgaccaa agaaactgac gcctcactta tccctcccct caccagaggt
     1081 c
//
