LOCUS       sequence-116956-         757 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Donor vector for mCFP knock-in via landing pad.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 757)
  AUTHORS   Ohtsuka M, Miura H, Mochida K, Hirose M, Hasegawa A, Ogura A, 
            Mizutani R, Kimura M, Isotani A, Ikawa M, Sato M, Gurumurthy CB
  TITLE     One-step generation of multiple transgenic mouse lines using an 
            improved Pronuclear Injection-based Targeted Transgenesis (i-PITT).
  JOURNAL   BMC Genomics. 2015 Apr 9;16:274. doi: 10.1186/s12864-015-1432-5.
  PUBMED    25887549
REFERENCE   2  (bases 1 to 757)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Genomics."; date: "2015-04-9"; volume: "16:274. doi"; pages: " 
            10.1186/s12864-015-1432-5"
COMMENT     Sequence Label: pBGV
FEATURES             Location/Qualifiers
     source          1..757
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    99..132
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     protein_bind    complement(141..174)
                     /label=loxJTZ17
                     /bound_moiety="Cre recombinase"
                     /note="Right element (RE) mutant of loxP (Araki et al., 
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      231..240
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(237..239,240..242,243..755)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHRFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTWGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYISHNVYITADKQKNGIK
                     AHFKIRHNIE"
     primer_bind     complement(282..303)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(543..562)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 ggtgagccag tgagttgatt gcagtccagt tacgctggag tctgaggctc gtcctgaatg
       61 atatgcggcc tcggatccgc gatcgatccg cggtgcgggt gccagggcgt gcccttgggc
      121 tccccgggcg cgtactccac ataacttcgt atagcataca ttatagcaat ttatcgaagt
      181 tcctattccg aagttcctat tctatcagaa gtataggaac ttcaccggtc gccaccatgg
      241 tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag ctggacggcg
      301 acgtaaacgg ccacaggttc agcgtgtccg gcgagggcga gggcgatgcc acctacggca
      361 agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg
      421 tgaccaccct gacctggggc gtgcagtgct tcagccgcta ccccgaccac atgaagcagc
      481 acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgtacc atcttcttca
      541 aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac accctggtga
      601 accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg gggcacaagc
      661 tggagtacaa ctacatcagc cacaacgtct atatcaccgc cgacaagcag aagaacggca
      721 tcaaggccca cttcaagatc cgccacaaca tcgagga
//
