LOCUS       sequence-116957-         810 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Donor vector for iRFP knock-in via landing pad.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 810)
  AUTHORS   Ohtsuka M, Miura H, Mochida K, Hirose M, Hasegawa A, Ogura A, 
            Mizutani R, Kimura M, Isotani A, Ikawa M, Sato M, Gurumurthy CB
  TITLE     One-step generation of multiple transgenic mouse lines using an 
            improved Pronuclear Injection-based Targeted Transgenesis (i-PITT).
  JOURNAL   BMC Genomics. 2015 Apr 9;16:274. doi: 10.1186/s12864-015-1432-5.
  PUBMED    25887549
REFERENCE   2  (bases 1 to 810)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Genomics."; date: "2015-04-9"; volume: "16:274. doi"; pages: " 
            10.1186/s12864-015-1432-5"
COMMENT     Sequence Label: pBGT
FEATURES             Location/Qualifiers
     source          1..810
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    100..133
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     protein_bind    complement(142..175)
                     /label=loxJTZ17
                     /bound_moiety="Cre recombinase"
                     /note="Right element (RE) mutant of loxP (Araki et al., 
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      232..241
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 aggtgagcca gtgagttgat tgcagtccag ttacgctgga gtctgaggct cgtcctgaat
       61 gatatgcggc ctcggatccg cgatcgatcc gcggtgcggg tgccagggcg tgcccttggg
      121 ctccccgggc gcgtactcca cataacttcg tatagcatac attatagcaa tttatcgaag
      181 ttcctattcc gaagttccta ttctatcaga agtataggaa cttcaccggt cgccaccatg
      241 gccgaaggga gcgtggcaag acagcccgat ctgctgacat gcgacgacga accaatccac
      301 attccaggag ctatccagcc tcatggactg ctgctggctc tggccgctga catgactatc
      361 gtggcaggct ctgataacct gccagagctg accgggctgg ctatcggagc actgattggc
      421 cggtccgcag ccgacgtgtt cgattctgag actcacaata gactgaccat cgccctggct
      481 gaacctgggg ctgcagtggg agccccaatt accgtcggct tcacaatgcg aaaggacgct
      541 ggctttatcg ggtcctggca ccgccatgat cagctgattt ttctggagct ggaaccacct
      601 cagagagacg tggcagagcc acaggctttc tttcggagaa caaacagcgc catcaggcgc
      661 ctgcaggccg ctgagactct ggaatccgct tgcgcagccg ctgcacagga agtgaggaag
      721 atcaccgggt tcgatcgcgt catgatctac agattcgcct cagactttag cggagaagtg
      781 atcgcagaag atcgctgtgc cgaggtcgaa
//
