LOCUS       sequence-116967-        1075 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Tribolium U6b promoter with BsaI cloning site to insert guide RNA 
            sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1075)
  AUTHORS   Gilles AF, Schinko JB, Averof M
  TITLE     Efficient CRISPR-mediated gene targeting and transgene replacement 
            in the beetle Tribolium castaneum.
  JOURNAL   Development. 2015 Jul 9. pii: dev.125054.
  PUBMED    26160901
REFERENCE   2  (bases 1 to 1075)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Development. 2015 Jul 9. pii: dev.125054."
COMMENT     Sequence Label: p(U6b-BsaI-gRNA)
FEATURES             Location/Qualifiers
     source          1..1075
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     rep_origin      complement(50..505)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(187..208)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     399..418
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     632..654
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     646..663
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     647..663
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     673..692
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        673..691
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_RNA        complement(742..817)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccaccta aattgtaagc
       61 gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt ttttaaccaa
      121 taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat agggttgagt
      181 gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa cgtcaaaggg
      241 cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta atcaagtttt
      301 ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc ccgatttaga
      361 gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc gaaaggagcg
      421 ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac acccgccgcg
      481 cttaatgcgc cgctacaggg cgcgtcccat tcgccattca ggctgcgcaa ctgttgggaa
      541 gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg cgaaaggggg atgtgctgca
      601 aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa aacgacggcc
      661 agtgagcgcg cgtaatacga ctcactatag ggcgaattgg gtacgaattc ccgggattaa
      721 gtggcgcgcc attacaaaaa agcaccgact cggtgccact ttttcaagtt gataacggac
      781 tagccttatt ttaacttgct atttctagct ctaaaactga gaccctctct cggtctctcg
      841 aacggtgctt ctacctgcct tgtatatgta gttagcggca accgatacgt agtagtgaga
      901 tataagagat ttgaaatttc ccgctttatt ttgattggtc ggttacaatc gatcgcaaaa
      961 cacaaacgtt cgtaatgatt cgtatatttg tatgtaatgg cgcttattta aaatttttat
     1021 tatttagttg aagaaatatt aatttgtcgt aatgaaaaca gttttgaaac aaaaa
//
