LOCUS       sequence-116968-        1087 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of G protein-coupled receptors for PRESTO-Tango: parallel
            receptorome expression and screening via transcriptional output, 
            with transcriptional activation following arrestin translocation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1087)
  AUTHORS   Kroeze WK, Sassano MF, Huang XP, Lansu K, McCorvy JD, Giguere PM, 
            Sciaky N, Roth BL
  TITLE     PRESTO-Tango as an open-source resource for interrogation of the 
            druggable human GPCRome.
  JOURNAL   Nat Struct Mol Biol. 2015 May;22(5):362-9. doi: 10.1038/nsmb.3014. 
            Epub 2015 Apr 20.
  PUBMED    25895059
REFERENCE   2  (bases 1 to 1087)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nsmb.3014";
            journalName: "Nat Struct Mol Biol"; date: "2015-05"; volume: "22"; 
            issue: "5"; pages: "362-9"
COMMENT     Sequence Label: C5A-Tango
FEATURES             Location/Qualifiers
     source          1..1087
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     46..65
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        46..64
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 tctctggcta actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta
       61 tagggagacc caagctggct agcgtttaaa cttaagcttg gtaccgagct cggatccact
      121 agtccagtgt ggtggaattc tgcagatatc cagcacagtg gcggccgcgc caccatgaag
      181 acgatcatcg ccctgagcta catcttctgc ctggtattcg ccgactacaa ggacgatgat
      241 gacgccagca tcgatatgaa ttcatttaac tatactaccc ctgattacgg gcactatgac
      301 gataaggaca ccctcgacct taatacccct gtcgataaga caagcaacac gctgagggtt
      361 cccgatatct tggcattggt cattttcgcc gtggtgttcc tggttggggt gctgggaaac
      421 gccttggtcg tgtgggttac agcatttgaa gctaaacgga ccatcaacgc aatttggttt
      481 ctgaatctcg cagtggccga ctttctgtcc tgtcttgcct tgcccatcct gttcacatct
      541 attgtgcagc accaccattg gccctttgga ggagccgcct gtagtatcct gccctctctg
      601 attctgctca acatgtacgc gtcaatactc cttctcgcaa ccattagcgc ggacaggttt
      661 ctcttggtgt tcaagcccat ttggtgccaa aatttccggg gggcgggcct cgcctggatt
      721 gcatgcgcgg tcgcatgggg tctggcattg ctcctcacta tcccgtcatt cctttacaga
      781 gttgttaggg aggaatactt cccaccaaag gtgctttgcg gtgtggacta cagccatgac
      841 aagcggagag agagggctgt ggcaatcgtg cggctcgtgc ttggcttttt gtggcccttg
      901 ctgacgctca caatttgtta cacatttata ttgctgagaa cctggtcaag gagagctacc
      961 cggagcacaa agacactgaa agtggtagta gccgtcgtgg caagcttctt tatattttgg
     1021 ctgccttatc aggtgactgg tattatgatg tcctttttgg agcccagctc cccaaccttc
     1081 ctgttgt
//
