LOCUS       sequence-116969-        1102 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of G protein-coupled receptors for PRESTO-Tango: parallel
            receptorome expression and screening via transcriptional output, 
            with transcriptional activation following arrestin translocation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1102)
  AUTHORS   Kroeze WK, Sassano MF, Huang XP, Lansu K, McCorvy JD, Giguere PM, 
            Sciaky N, Roth BL
  TITLE     PRESTO-Tango as an open-source resource for interrogation of the 
            druggable human GPCRome.
  JOURNAL   Nat Struct Mol Biol. 2015 May;22(5):362-9. doi: 10.1038/nsmb.3014. 
            Epub 2015 Apr 20.
  PUBMED    25895059
REFERENCE   2  (bases 1 to 1102)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nsmb.3014";
            journalName: "Nat Struct Mol Biol"; date: "2015-05"; volume: "22"; 
            issue: "5"; pages: "362-9"
COMMENT     Sequence Label: APJ-Tango
FEATURES             Location/Qualifiers
     source          1..1102
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     46..65
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        46..64
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 tctctggcta actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta
       61 tagggagacc caagctggct agcgtttaaa cttaagcttg gtaccgagct cggatccact
      121 agtccagtgt ggtggaattc tgcagatatc cagcacagtg gcggccgcgc caccatgaag
      181 acgatcatcg ccctgagcta catcttctgc ctggtattcg ccgactacaa ggacgatgat
      241 gacgccagca tcgatatgga ggagggcgga gactttgaca attattacgg tgccgataat
      301 cagtccgagt gcgagtacac tgactggaaa agctccggtg cactcatccc tgctatatat
      361 atgctggtgt ttctgttggg aaccactgga aacggcctcg tactgtggac tgtgtttagg
      421 agttcccggg aaaaacggag gagcgccgat atttttattg caagcctggc cgtggctgac
      481 cttacgtttg tggtgaccct gccactgtgg gcaacctaca catatcgcga ttacgattgg
      541 cctttcggaa cattcttctg caaactgagt tcttatctca tcttcgttaa tatgtatgca
      601 tctgtcttct gtctgacggg cctcagtttt gatcggtacc tcgcgattgt tcggcccgta
      661 gctaatgcca gactgaggct gagagtcagt ggggccgtcg ccacagcagt gctctgggtg
      721 ttggcggctc tgcttgcgat gccagtaatg gtactcagga caaccggaga cctggagaac
      781 actacaaaag tgcagtgtta tatggattat agcatggtcg ctaccgtctc aagcgagtgg
      841 gcttgggaag tggggttggg cgtgagcagc actacagttg ggttcgtggt acccttcacg
      901 attatgctga catgctactt cttcattgcc cagaccatcg ctggtcactt ccggaaagag
      961 aggattgaag gcctccggaa gcggaggcgg ctgctgagca tcattgttgt gctggtcgtg
     1021 acctttgctc tctgttggat gccgtatcat ctggtgaaaa cactctacat gctcggatcc
     1081 ctgttgcact ggccctgcga tt
//
