LOCUS       sequence-116971-         685 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Yeast expression plasmid for roGFP2-Grx1; yeast codon optimized 
            roGFP; yeast Grx1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 685)
  AUTHORS   Morgan B, Ezerina D, Amoako TN, Riemer J, Seedorf M, Dick TP
  TITLE     Multiple glutathione disulfide removal pathways mediate cytosolic 
            redox homeostasis.
  JOURNAL   Nat Chem Biol. 2013 Feb;9(2):119-25. doi: 10.1038/nchembio.1142. 
            Epub 2012 Dec 16.
  PUBMED    23242256
REFERENCE   2  (bases 1 to 685)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nchembio.1142"; journalName: "Nat Chem Biol"; date: 
            "2013-02"; volume: "9"; issue: "2"; pages: "119-25"
COMMENT     Sequence Label: p415TEF cyto roGFP2-Grx1(yeast)
FEATURES             Location/Qualifiers
     source          1..685
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(8..197)
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     209..227
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     245..261
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     246..262
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 ttaaagcctt cgagcgtccc aaaaccttct caagcaaggt tttcagtata atgttacatg
       61 cgtacacgcg tctgtacaga aaaaaaagaa aaatttgaaa tataaataac gttcttaata
      121 ctaacataac tataaaaaaa taaataggga cctagacttc aggttgtcta actccttcct
      181 tttcggttag agcggatgtg gggggagggc gtgaatgtaa gcgtgacata actaattaca
      241 tgactcgagg tcgacggtat cgataagctt ttaatttgca agaataggtt ctaacaattc
      301 ctccaattca ccagtctccc tcaattcctg caagtcgtcg ttgcctccaa tatgtttacc
      361 attaatatag atgtttggca cggttctttg gccattaatc tcatataacg cagcctgaat
      421 gtctgcgcct tccttcatgt cattcaattg caaaaccaga actttggacc tgggaacctt
      481 taacttttca aaaagcgtgt ttagggctgc atggcagtat ggacagtacg ttttggatgc
      541 gacgaagatc tcgttttctg caataaggtc cttgacgtgc ttgatagttt cttgagatac
      601 catggtgaat tctcctcctg atcctcctcc tcctgatcct cctcctcctg atcctcctcc
      661 acctgaacca ccaccacctg aacca
//
