LOCUS       sequence-116974-        1051 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of G protein-coupled receptors for PRESTO-Tango: parallel
            receptorome expression and screening via transcriptional output, 
            with transcriptional activation following arrestin translocation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1051)
  AUTHORS   Kroeze WK, Sassano MF, Huang XP, Lansu K, McCorvy JD, Giguere PM, 
            Sciaky N, Roth BL
  TITLE     PRESTO-Tango as an open-source resource for interrogation of the 
            druggable human GPCRome.
  JOURNAL   Nat Struct Mol Biol. 2015 May;22(5):362-9. doi: 10.1038/nsmb.3014. 
            Epub 2015 Apr 20.
  PUBMED    25895059
REFERENCE   2  (bases 1 to 1051)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nsmb.3014";
            journalName: "Nat Struct Mol Biol"; date: "2015-05"; volume: "22"; 
            issue: "5"; pages: "362-9"
COMMENT     Sequence Label: CHRM1-Tango
FEATURES             Location/Qualifiers
     source          1..1051
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     46..65
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        46..64
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 tctctggcta actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta
       61 tagggagacc caagctggct agcgtttaaa cttaagcttg gtaccgagct cggatccact
      121 agtccagtgt ggtggaattc tgcagatatc cagcacagtg gcggccgcgc caccatgaag
      181 acgatcatcg ccctgagcta catcttctgc ctggtattcg ccgactacaa ggacgatgat
      241 gacgccagca tcgatatgaa tacaagcgct cctccagcgg tttccccaaa tattacggtg
      301 ctggcacctg ggaaaggacc atggcaagtt gcttttatcg gtatcaccac cggcttgctc
      361 tccttggcaa cagtcacggg caatttgctc gtactgatct catttaaggt gaacacagag
      421 ctgaaaacag tgaacaacta cttcttgttg agtttggcct gtgcggacct tattatcgga
      481 acgttttcaa tgaatctgta tacaacctat ctcctgatgg gccactgggc gctgggaacc
      541 cttgcttgcg acctttggtt ggcccttgat tacgtggcga gcaacgcaag cgtcatgaat
      601 ctcctgttga tatcatttga caggtatttt tctgttaccc ggcccctgag ctacagagct
      661 aaacgaactc cacgacgcgc tgcactgatg attggtctgg cctggctggt cagttttgtc
      721 ctgtgggccc ctgccatact gttttggcag tacctggtgg gagagcgcac cgtgcttgca
      781 ggtcagtgct atatacagtt tctgtctcag cccataatca cattcggcac cgcgatggcg
      841 gcattttact tgcctgtgac tgttatgtgt actctgtatt ggcgaattta ccgcgaaacc
      901 gagaatagag ccagggaact tgctgcactg cagggttcag agacacccgg aaagggcggc
      961 ggctccagta gtagcagtga gagaagtcaa cctggggctg aagggtcccc cgagactccc
     1021 ccaggaaggt gctgccgatg ctgcagggcc c
//
