LOCUS       sequence-116977-        1076 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of G protein-coupled receptors for PRESTO-Tango: parallel
            receptorome expression and screening via transcriptional output, 
            with transcriptional activation following arrestin translocation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1076)
  AUTHORS   Kroeze WK, Sassano MF, Huang XP, Lansu K, McCorvy JD, Giguere PM, 
            Sciaky N, Roth BL
  TITLE     PRESTO-Tango as an open-source resource for interrogation of the 
            druggable human GPCRome.
  JOURNAL   Nat Struct Mol Biol. 2015 May;22(5):362-9. doi: 10.1038/nsmb.3014. 
            Epub 2015 Apr 20.
  PUBMED    25895059
REFERENCE   2  (bases 1 to 1076)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nsmb.3014";
            journalName: "Nat Struct Mol Biol"; date: "2015-05"; volume: "22"; 
            issue: "5"; pages: "362-9"
COMMENT     Sequence Label: CRHR1-Tango
FEATURES             Location/Qualifiers
     source          1..1076
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     46..65
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        46..64
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 tctctggcta actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta
       61 tagggagacc caagctggct agcgtttaaa cttaagcttg gtaccgagct cggatccact
      121 agtccagtgt ggtggaattc tgcagatatc cagcacagtg gcggccgcgc caccatgaag
      181 acgatcatcg ccctgagcta catcttctgc ctggtattcg ccgactacaa ggacgatgat
      241 gacgccagca tcgatatggg aggtcatccg cagctgcggc ttgttaaggc tctcctgctg
      301 ctgggcctca accctgtcag tgccagcctc caggaccagc actgtgagtc tctcagtctg
      361 gccagcaata taagtggact gcagtgtaac gcctcagtgg acttgatagg cacttgctgg
      421 cctcgaagtc ctgctggtca gctggtggta cgaccctgcc cagcattctt ctatggcgtg
      481 cgatataaca ccacgaataa tgggtaccgc gaatgcctgg ccaacggcag ttgggccgca
      541 agggtgaact attctgaatg ccaggaaatt ctgaatgaag aaaagaagag taaggttcac
      601 taccacgtgg ccgtgatcat caactatctc ggtcactgta tctcactggt tgccttgttg
      661 gttgcattcg tactgttttt gaggctgaga cctggctgta cacactgggg agaccaggct
      721 gatggagccc tggaggtggg ggccccttgg tccggagcac cttttcaagt ccgacgcagc
      781 atccggtgcc tgcgcaacat aatccactgg aaccttatat ccgctttcat tctgaggaac
      841 gctacatggt tcgtggtgca gttgactatg tctcctgagg tccatcagtc caacgttggc
      901 tggtgtaggc tggtcacagc tgcatacaat tacttccatg tgaccaattt cttttggatg
      961 ttcggtgaag ggtgctatct ccacacggca atagttctga cgtactcaac agaccgactt
     1021 agaaaatgga tgttcatctg cattgggtgg ggtgtcccat ttcccatcat cgttgc
//
