LOCUS       sequence-116997-        9954 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9954)
  AUTHORS   Srinivas S, Watanabe T, Lin CS, William CM, Tanabe Y, Jessell TM, 
            Costantini F
  TITLE     Cre reporter strains produced by targeted insertion of EYFP and ECFP
            into the ROSA26 locus.
  JOURNAL   BMC Dev Biol. 2001;1:4. doi: 10.1186/1471-213x-1-4. Epub 2001 Mar 
            27.
  PUBMED    11299042
REFERENCE   2  (bases 1 to 9954)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Dev 
            Biol."; date: "2001"; volume: "1:4. doi"; pages: " 
            10.1186/1471-213x-1-4. Epub 2001 Mar 27"
COMMENT     Sequence Label: pROSA26-PA
FEATURES             Location/Qualifiers
     source          1..9954
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    107..128
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(143..148,149..166,167..173)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    181..197
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     186..208
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     205..221
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     205..221
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     240..260
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        242..260
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     polyA_signal    322..546
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     535..552
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     intron          620..685
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             complement(762..1334)
                     /codon_start=1
                     /gene="Corynebacterium diphtheriae tox"
                     /product="diphtheria toxin fragment A"
                     /label=DTA
                     /translation="DDVVDSSKSFVMENFSSYHGTKPGYVDSIQKGIQKPKSGTQGNYD
                     DDWKGFYSTDNKYDAAGYSVDNENPLSGKAGGVVKVTYPGLTKVLALKVDNAETIKKEL
                     GLSLTEPLMEQVGTEEFIKRFGDGASRVVLSLPFAEGSSSVEYINNWEQAKALSVELEI
                     NFETRGKRGQDAMYEYMAQACAGNRVRR"
     promoter        complement(1361..1860)
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(1409..1428)
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     1802..1825
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     misc_feature    1910..6250
                     /label=Rosa26 right arm
                     /note="right homology arm from intron 1 of the mouse Rosa26
                     gene (Soriano, 1999)"
     misc_feature    6278..7363
                     /label=Rosa26 left arm
                     /note="left homology arm from intron 1 of the mouse Rosa26 
                     gene (Soriano, 1999)"
     primer_bind     complement(7381..7400)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(7382..7400)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(7410..7427)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(7410..7426)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7419..7441)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      complement(7567..8022)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7704..7725)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     7916..7935
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     promoter        8049..8153
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(8154..8222,8223..9014)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(8372..8391)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      9185..9773
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     9674..9693
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     9927..9944
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg
      241 caattaaccc tcactaaagg gaacaaaagc tgggtaccgg gccccccctc gagccccagc
      301 tggttctttc cgcctcagaa gccatagagc ccaccgcatc cccagcatgc ctgctattgt
      361 cttcccaatc ctcccccttg ctgtcctgcc ccaccccacc ccccagaata gaatgacacc
      421 tactcagaca atgcgatgca atttcctcat tttattagga aaggacagtg ggagtggcac
      481 cttccagggt caaggaaggc acgggggagg ggcaaacaac agatggctgg caactagaag
      541 gcacagtcga ggctgatcag cgagctctag gatctgcatt ccaccactgc tcccattcat
      601 cagttccata ggttggaatc taaaatacac aaacaattag aatcagtagt ttaacacatt
      661 atacacttaa aaattttata tttaccttag agctttaaat ctctgtaggt agtttgtcca
      721 attatgtcac accacagaag taaggttcct tcacaaagag atcgcctgac acgatttcct
      781 gcacaggctt gagccatata ctcatacatc gcatcttggc cacgttttcc acgggtttca
      841 aaattaatct caagttctac gcttaacgct ttcgcctgtt cccagttatt aatatattca
      901 acgctagaac tcccctcagc gaagggaagg ctgagcacta cacgcgaagc accatcaccg
      961 aaccttttga taaactcttc cgttccgact tgctccatca acggttcagt gagacttaaa
     1021 cctaactctt tcttaatagt ttcggcatta tccactttta gtgcgagaac cttcgtcagt
     1081 cctggatacg tcactttgac cacgcctcca gcttttccag agagcgggtt ttcattatct
     1141 acagagtatc ccgcagcgtc gtatttattg tcggtactat aaaacccttt ccaatcatcg
     1201 tcataatttc cttgtgtacc agattttggc ttttgtatac ctttttgaat ggaatctaca
     1261 taaccaggtt tagtcccgtg gtacgaagaa aagttttcca tcacaaaaga tttagaagaa
     1321 tcaacaacat catcaggatc catggcgagg acctgcaggt cgaaaggccc ggagatgagg
     1381 aagaggagaa cagcgcggca gacgtgcgct tttgaagcgt gcagaatgcc gggcctccgg
     1441 aggaccttcg ggcgcccgcc ccgcccctga gcccgcccct gagcccgccc ccggacccac
     1501 cccttcccag cctctgagcc cagaaagcga aggagcaaag ctgctattgg ccgctgcccc
     1561 aaaggcctac ccgcttccat tgctcagcgg tgctgtccat ctgcacgaga ctagtgagac
     1621 gtgctacttc catttgtcac gtcctgcacg acgcgagctg cggggcgggg gggaacttcc
     1681 tgactagggg aggagtagaa ggtggcgcga aggggccacc aaagaacgga gccggttggc
     1741 gcctaccggt ggatgtggaa tgtgtgcgag gccagaggcc acttgtgtag cgccaagtgc
     1801 ccagcggggc tgctaaagcg catgctccag actgccttgg gaaaagcgcc tcccctaccc
     1861 ggtagaattc gatatcaagc ttatcgatac cgtcgacggt atcgataagc tagatgtcct
     1921 gaaatatttc tatcagaaca aggtagtata aagctggtag gtatacaaaa cgctagacta
     1981 gtttctatcc ctgaccctta atctgctagt atatccgtag gaagttgctt aagtgccact
     2041 agtaccaaca gcctctgtta gagtgaacag tagattttaa tgaagggcca ataaagacca
     2101 cttaagacta ctgactgaat taaaaatcca cccagtggag ttagtattgg ttatagttat
     2161 ataacaaaat atccaactta gccaggcgtg gtggtgcacg cctttaatcc cggcactcag
     2221 gaggcagagg caggcggatt tctgagttcg aggccagcct ggtctacaaa gtgagttcca
     2281 ggacagccag ggctatacag agaaaccctg tctcgaaaaa ccaaaaaaaa aaaaaaaaag
     2341 gaaaaaaaat atggcttgaa aacatgctat tgttgtcaaa aggagatcca aacaagtatt
     2401 tctttgaaca aaaattatgt agaaaacagg gtgataaaga acaagggctt tttaaaaaag
     2461 gccagcatta tttgagatat acaagtgact ccaattcaag cctaaaggcc actcaatgct
     2521 cactaacagt gctaatcaga atcaattcct tagcccagtc aaaaatagtc ctgaatatct
     2581 tctggataaa catgtcctcc aacatataaa ctagtataca ctaacaaaac gtctcaactt
     2641 caaggtgaaa tgcttgactc ctagacttgt gacccagcaa agtgctctat aggtagggtt
     2701 actaggtcag agagtcttgc ctgcaaacca caattataca attccaccaa atgtaagaca
     2761 tgtaaaaatg acttatttag ctcaggtttt gaaaattaaa aatcaaaccc acaaagacct
     2821 aactttcaca ttaagtacaa atgtttaaca tatataacat gttttgcctt gatatatgaa
     2881 atcatatgac atcacctgac aggaaactta agtttatttg aacaacaatc agcctaaggt
     2941 aggcctagca catgatcagg atagtgcagg gaaacccaaa gaagtgcttc tgagtataaa
     3001 ccaagagacc tcgaaatagc agctttgttc tgtatctcat gggctttaac tatcacttga
     3061 aaacaatttc acaaatcaca aaaccaacat ttgtttagtt tccaactctg cctattcacc
     3121 ggagaaatcc atgtcccaat ttgatggggg aaaacttgaa tgaaatatta tttggaatat
     3181 tttaatatcc ctgtgtatat gcagatggtt taaagacagg taaccttaac tgtagtttct
     3241 tggttgtctg ccttgttttt tgaaatatgc tctcaccagg agcctgccaa gtaactactc
     3301 ttgtgtgctc agcaagtcct agggatccct taagcatgct ctaacaggcc tggctttttt
     3361 ttttaaaaag taggttgcgg tgataataat tcaagttttc atcacttgaa acacatttta
     3421 ccaactatca cccaagctcc cttagtgtgt cccctataaa agaatctgac ctgcaagttc
     3481 caaaagtatt actacatttt aaccttagta ttcaggagac aaaagcaggc agaactttag
     3541 tctacatatc ttgttgcaga tcaggcagag atacatagtg agaccctgcc tcaggaaaag
     3601 ctatcatttt tatattcaca gtaagcaaat taacattaaa agtcagaaat tcttaatttg
     3661 actttggctg tgaagaattt ggattctctg gatttgaaac tagaaaatct gattaagcga
     3721 tgcaacagtt tctgtatact aaaactctaa gacccttggt tctaaagata ccacatttaa
     3781 aaaaaaagtt aagtataaga caaaaagcct taatagctct tttatagggg agggactcat
     3841 ttaatattag tccacctcac tcctcataac tattttatga ggtttattat ataacccatt
     3901 ttttaaaaaa aaaattaaag gacaatttta gtgtttgaaa gatttcccaa ccccaccctg
     3961 gaaatcaggc tgcaaatctc agcagctgcc cttagaaggc aaaaagccat gccatttaag
     4021 ccatgggaag ttagtagcaa acaagagacc acatggcagg aaggaggctt taaagaaagc
     4081 ccacagtgtc aaaagaccca accaacagca gagacagaaa aaataatacc caaatctcat
     4141 cagaaaggta gacggattta gccacatcca tagtggctca ttagggaatg cttcaattat
     4201 aattttatga aatctcagag cccccctccc caacagggtt tctgtgtgta ttcctggcta
     4261 tcctagatag aacttgatgt gtagaccagg ctgggctcaa actcagagat ctgcctgtct
     4321 ctgcctccag agtgctggga ttaaaggcat aaaggcaagc accaccactg gctggctaaa
     4381 ctctggccct acatttttga aatccaaaat tatcattact tgctttcatg tttagtaatg
     4441 gctgcagact tagctttcag ctttgtatag atgaagcaca aaagttaagt aaagctagca
     4501 gtttaaacaa ataggatact agaagttcaa tttttatttc tttattgctt agtgtaagag
     4561 aaaagacatt aaagtctgca ttggtcttct gtatcccaca agtctgcagt tatggctcct
     4621 ctgtccacag ttacacttca aataaaaatt agttccttgg taacatttcc agtgcttaaa
     4681 gagtaatttg attattggct accatattgg aacaaacaca aagtatttca ttacttcagt
     4741 aaattctact ggagacagta ttctctttaa aaaaaaaaaa aagaagaaga aggaggattt
     4801 atttatttaa tgtgaataca cttgtggtct tcagacacac cagaagaggg catcagatcc
     4861 cattacagat ggttgtgagc caccatgtgg ttgctgggaa ttgaactcag gacttctgga
     4921 agagcagtct gtgctcttga actccgagcc acctctccag cccctggata gtattattat
     4981 agagcacaag cacacacaac acaacaacct aaaaaccaag cttcacaaat acatttgttg
     5041 cttaatcatt ttacttgtgt ttggctacaa gtcaagcaaa attataggtc ctgaagaagc
     5101 ttggcaaaat cacatttaga ccagcaataa cgtgtagaat gccatgagtc aagccagtcc
     5161 aagagaaagc actgcttaaa tagaaaaaat acaagctcac aagaccttag gtcaggaaag
     5221 acaacaaaca cctgaacttt gcattccaaa aggaaccacc ttttacagat gtgtacaatg
     5281 acaaaatttt agtaagcagt aatcaatacc atgtggctca ataatgaaat atacctttta
     5341 atgtcttaaa atatcagcct ggcaatatgt aagatacatc aggtaatatt gggggaggag
     5401 acatccacct ggaaaccatt aatggttaat agctcagttt ataaatggag aaaaaggaga
     5461 gaggcattca tgggagtgga aagttaagct ttaggttgga ttctcaatac atctattgtt
     5521 gtaaaactcc ctggactgag aataggccca aatgtggaac accacctgac gggagaggtg
     5581 atagacactg aaaattaagg atcaaggcaa aggatcaaca aaaagtgtac taaggagtta
     5641 taaaagaact gcagtgttga ggccccagct acagcctcga tttgtggtgt atgtaactaa
     5701 tctgtctggt ttcatgagtc atcagacttc taagatcagg aaagggaaaa tgccaatgct
     5761 ctgtctaggg gttggataag ccagtataat aaatgaaaat ggggctaaaa tgagtgttct
     5821 aaaatacctt ttgataaggc tgcagaagga gcgggagaaa tggatatgaa gtactgggct
     5881 ctttaaaaat gattaaaatt ctgcttacat agtctaactc gcgacactgt aatttcatac
     5941 tgtagtaagg atctcaagca ggagagtata aaactcgggt gagcatgtct ttaatctacc
     6001 tcgatggaaa atactccgag gcggatcaca agcaataata acctgtagtt ttgctgcata
     6061 aaaccccaga tgactaccta tcctcccatt ttccttattt gcccctatta aaaaacttcc
     6121 cgacaaaacc gaaaatctgt gggaagtctt gtccctccaa ttttacacct gttcaattcc
     6181 cctgcaggac aacgcccaca caccaggtta gcctttaagc ctgcccagaa gactcccgcc
     6241 catcttctag ccggcgcgcc atttaaatta attaaggcta gaaagactgg agttgcagat
     6301 cacgagggaa gagggggaag ggattctccc aggcccaggg cggtcctcag aagccaggag
     6361 gcagcagaga actcccagaa aggtattgca acactcccct cccccctccg gagaagggtg
     6421 cggccttctc cccgcctact ccactgcagc tcccttactg ataacaactc agagcgactt
     6481 tgggagagca agtgcttcct gcctccaaaa cagcccaact gagccctcgc tccttccctc
     6541 cactccccgg agtgcgcgat ggaggtctgg ctcagcacgc ccctcttgag gcaactcaag
     6601 tcggaaacgt gcttgcaccc gccccgcagc cgctcagccc tactgcccgt ccccgccccc
     6661 agcgcgcgct tcctgccacg ttgcgcaggg gcgcgcggcc agactctgcg gcgcggggcc
     6721 gaggggaggg ccggaacctg ggagcgcctc ctcgccgccc ccgctggccg gcggatggac
     6781 tcaacttgca cgaacacgag ccaatggcaa gggccagttt tctgggcccc gagagccaat
     6841 cagacgacga ggcccggccg gcggcgggta aaacgactcc cccagaggaa ggggagggtg
     6901 ggcggccgct cgcgcgcgag ctactttcgc tgaccctccc tcccctcccc cgccccgcca
     6961 gaggccgacc gcgcccgcac gtccagctcg cctcacccca cctacctccc gccccaccca
     7021 gtgggcagag cgaggctgcc ggcggctgcg cactccggct gccgttaact gacaggcgcc
     7081 ttacgccaac caaaacacgc catttgtgtt ttcacacacg gcgggaggaa aagaagccaa
     7141 tcagcgacga gacgtcggcc ggaagcgctc ctccgctgcc cccccccccc ccccgagcca
     7201 tggccgcgtc cggtggagac ttttccgctc ccttctccct ccccctccgg ttgctgcagg
     7261 gcggaccgca ttcctgccca ccacccgctt gccccttcca gcgtcacgac tcgtacccgg
     7321 ctgtctcaca gaacggctcc accacgctcg gagggcctgc cgcggtggag ctccaattcg
     7381 ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg
     7441 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
     7501 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
     7561 gaatggaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt tgttaaatca
     7621 gctcattttt taaccaatag gccgaaatcg gcaaaatccc ttataaatca aaagaataga
     7681 ccgagatagg gttgagtgtt gttccagttt ggaacaagag tccactatta aagaacgtgg
     7741 actccaacgt caaagggcga aaaaccgtct atcagggcga tggcccacta cgtgaaccat
     7801 caccctaatc aagttttttg gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag
     7861 ggagcccccg atttagagct tgacggggaa agccggcgaa cgtggcgaga aaggaaggga
     7921 agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa
     7981 ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc gtcaggtggc acttttcggg
     8041 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     8101 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     8161 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     8221 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     8281 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     8341 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     8401 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     8461 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     8521 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     8581 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     8641 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     8701 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     8761 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     8821 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     8881 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     8941 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     9001 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     9061 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     9121 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     9181 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     9241 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     9301 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     9361 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     9421 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     9481 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     9541 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     9601 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     9661 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     9721 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     9781 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     9841 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     9901 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaag
//
