LOCUS       sequence-116999-        1095 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of G protein-coupled receptors for PRESTO-Tango: parallel
            receptorome expression and screening via transcriptional output, 
            with transcriptional activation following arrestin translocation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1095)
  AUTHORS   Kroeze WK, Sassano MF, Huang XP, Lansu K, McCorvy JD, Giguere PM, 
            Sciaky N, Roth BL
  TITLE     PRESTO-Tango as an open-source resource for interrogation of the 
            druggable human GPCRome.
  JOURNAL   Nat Struct Mol Biol. 2015 May;22(5):362-9. doi: 10.1038/nsmb.3014. 
            Epub 2015 Apr 20.
  PUBMED    25895059
REFERENCE   2  (bases 1 to 1095)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nsmb.3014";
            journalName: "Nat Struct Mol Biol"; date: "2015-05"; volume: "22"; 
            issue: "5"; pages: "362-9"
COMMENT     Sequence Label: DRD5-Tango
FEATURES             Location/Qualifiers
     source          1..1095
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     46..65
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        46..64
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 tctctggcta actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta
       61 tagggagacc caagctggct agcgtttaaa cttaagcttg gtaccgagct cggatccact
      121 agtccagtgt ggtggaattc tgcagatatc cagcacagtg gcggccgcgc caccatgaag
      181 acgatcatcg ccctgagcta catcttctgc ctggtattcg ccgactacaa ggacgatgat
      241 gacgccagca tcgatatgtt gccaccaggg agcaatggta cggcgtatcc cgggcagttc
      301 gccctctacc aacaacttgc ccagggtaat gccgtcgggg gcagcgcagg cgctccacct
      361 ttggggccaa gtcaggtcgt gactgcctgt cttctgaccc ttctgataat atggactctg
      421 ctgggcaacg tcctggtttg tgcagcaatt gtgcgctctc gccacctgcg agctaacatg
      481 acaaacgtgt tcatagtgag cctggcagtg agcgacctgt tcgttgcgct cctcgtgatg
      541 ccctggaagg cggtggcaga agtggccgga tattggcctt ttggcgcatt ttgcgatgtt
      601 tgggtggcct ttgatattat gtgttccacg gccagtattc tcaatctgtg cgtgatttcc
      661 gtcgatcgct actgggcaat tagcaggccg ttccgctata aaagaaagat gacccagagg
      721 atggcccttg tgatggtcgg actggcctgg accctctcca tcctgatttc tttcattcct
      781 gttcagttga attggcacag ggaccaggct gcaagttggg gcgggctgga tctgccaaat
      841 aacctggcca attggacccc atgggaggaa gatttctggg aaccagacgt caacgcagag
      901 aattgtgata gtagccttaa tcggacctat gctattagtt ccagtctgat ctccttctac
      961 attccggtcg ctataatgat agtgacttac acaaggatct atagaatcgc tcaagttcaa
     1021 atacgaagga tctctagcct ggagcgcgcc gctgagcacg ctcaatcctg ccgctcatcc
     1081 gctgcttgtg ctccc
//
