LOCUS       sequence-117-exp        4970 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4970)
  AUTHORS   Fire A et al
  TITLE     Fire Lab C. Elegans Vector Kit 1995
REFERENCE   2  (bases 1 to 4970)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pPD52_37
FEATURES             Location/Qualifiers
     source          1..4970
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(2544..2566)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     2666..2685
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     complement(2763..2781)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        2849..2953
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2954..3022,3023..3814)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3172..3191)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3985..4573
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4474..4493
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4727..4744
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4861..4882
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4897..4902,4903..4920,4921..4927)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4935..4951
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4940..4962
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     join(4959..4970,1..5)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     join(4959..4970,1..5)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 atgaccatga ttacgccaag cttgcatgcc tgcaggaatt ccccggatcc agattcgaac
       61 cagcaatgac tctactgaag agcgcgcgct ttaccacttc tccaaacagg cgagtggcgg
      121 ccgcggttcc aaggtatatg tgaaaccaac cgagctgtac gaagtaggaa gcttccatta
      181 ctttttcaga tatttagttc tgaaaacttt gttaactctc cttatcaaca actaaatggc
      241 cctttttgta gttatttcta ctggaaaaaa atgtgcctgt aaataatatc ctgtttataa
      301 acgttgttgt ccagtgaaaa cattcccttg acctgaaatt tatcgataaa agataaattt
      361 atcttgaaaa ctactttttc gagtgtattt ttaaacattc tacaaattcc acttaatagc
      421 aggtcgggaa cttcggcgat tcctcgaata agctattttc aaaaaaaaaa ttaggcccaa
      481 aatttgtttc tgaaaaacga ccccaatctt gtattttcca gtaataaata caatctgaat
      541 ttttttcgaa attatttttc gcatcgaaat tggaaaacgt agtaaaaaat acggccgtag
      601 taaaagagta aaccgtagta aaagagcaac gtagtaaaag atgaggtagt aaaagagcat
      661 ttgcctaatt tcttttattt ttcacattat atgtccagtt aaaaaaaaag aactctgaga
      721 aataaatttt gaaaatcagg taaaaaaagt tagatgtttc agacgtcacc ttgttgttaa
      781 tatggtctct catatgattg gtgacatcag aatttttaac ttggaattga acattttaat
      841 tcttgattta ttattttcaa gatttaattt taagttttaa aaatagattt ttttttcaaa
      901 aaaaaaacaa ggttgtaaaa attgttaaaa ctggaaacat cttttagcta tttttatttt
      961 agcctgcaag aattgctcct gtggagaaac gtgtaagtgt accgtcgaaa aatgttgctg
     1021 cgaggaagag aaaaaatcgt gctgtgaagg aaaaaccgag aagactgagc aaaagaaata
     1081 accaatgagc aagtgaactt ttcccacgtc tactactaaa ttattgtcga tctataattc
     1141 tttcgcttat caatcttgtc aattgaaata aaacaatatt tttctaattc tttttggaac
     1201 gaacacacgt gtttaaatga atgttgtgct aaaaacgtca catcaatggt acgtgaatgt
     1261 tgcaaacacc ttgtcaataa ctgataaaat cagaaactag agctgtgact gaatcgtata
     1321 ctagaacgga gtctctctaa aaacgttcta aaaacaaaca aaaaattgtg caaaggatta
     1381 gagtgctcaa gatcaatgag caaactcaca atcaactatc tgttattgtt ttgggtctct
     1441 tctatatctc tattctttta gtatcataag tttgtacatt gtgacagggc caccctcttt
     1501 tatcacatat ttgaagtggt aaacagccag caaaaaccaa ataaaaaggc agtgagaaaa
     1561 agaagaaggc agctcaattt gactgctgaa attaagaact ctctagagga tccccgggat
     1621 tggccaaagg acccaaaggt atgtttcgaa tgatactaac ataacataga acattttcag
     1681 gaggaccctt ggctagcgtc gacggtacca tggtattgat atctgagctc cgcatcggcc
     1741 gctgtcatca gatcgccatc tcgcgcccgt gcctctgact tctaagtcca attactcttc
     1801 aacatcccta catgctcttt ctccctgtgc tcccaccccc tatttttgtt attatcaaaa
     1861 aacttctctt aatttctttg ttttttagct tcttttaagt cacctctaac aatgaaattg
     1921 tgtagattca aaaatagaat taattcgtaa taaaaagtcg aaaaaaattg tgctccctcc
     1981 ccccattaat aataattcta tcccaaaatc tacacaatgt tctgtgtaca cttcttatgt
     2041 tttttacttc tgataaattt ttttgaaaca tcatagaaaa aaccgcacac aaaatacctt
     2101 atcatatgtt acgtttcagt ttatgaccgc aatttttatt tcttcgcacg tctgggcctc
     2161 tcatgacgtc aaatcatgct catcgtgaaa aagttttgga gtatttttgg aatttttcaa
     2221 tcaagtgaaa gtttatgaaa ttaattttcc tgcttttgct ttttggggtt tcccctattg
     2281 tttgtcaaga tttcgaggac ggcgtttttc ttgctaaaat cacaagtatt gatgagcacg
     2341 atgcaagaaa gatcggaaga aggtttgggt ttgaggctca gtggaaggtg agtagaagtt
     2401 gataatttga aagtggagta gtgtctatgg ggtttttgcc ttaaatgaca gaatacattc
     2461 ccaatatacc aaacataact gtttcctact agtcggccgt acgggccctt tcgtctcgcg
     2521 cgtttcggtg atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct
     2581 tgtctgtaag cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc
     2641 gggtgtcggg gctggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat
     2701 atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag gcggccttaa
     2761 gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac
     2821 gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     2881 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     2941 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
     3001 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
     3061 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
     3121 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
     3181 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
     3241 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
     3301 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
     3361 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
     3421 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
     3481 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
     3541 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
     3601 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
     3661 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
     3721 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
     3781 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
     3841 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     3901 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     3961 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     4021 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     4081 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt
     4141 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     4201 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     4261 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     4321 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagcattg
     4381 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     4441 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     4501 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     4561 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     4621 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
     4681 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
     4741 cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca
     4801 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat
     4861 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
     4921 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct
//
