LOCUS       sequence-117012-        1103 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of G protein-coupled receptors for PRESTO-Tango: parallel
            receptorome expression and screening via transcriptional output, 
            with transcriptional activation following arrestin translocation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1103)
  AUTHORS   Kroeze WK, Sassano MF, Huang XP, Lansu K, McCorvy JD, Giguere PM, 
            Sciaky N, Roth BL
  TITLE     PRESTO-Tango as an open-source resource for interrogation of the 
            druggable human GPCRome.
  JOURNAL   Nat Struct Mol Biol. 2015 May;22(5):362-9. doi: 10.1038/nsmb.3014. 
            Epub 2015 Apr 20.
  PUBMED    25895059
REFERENCE   2  (bases 1 to 1103)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nsmb.3014";
            journalName: "Nat Struct Mol Biol"; date: "2015-05"; volume: "22"; 
            issue: "5"; pages: "362-9"
COMMENT     Sequence Label: CALCRb-Tango
FEATURES             Location/Qualifiers
     source          1..1103
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     46..65
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        46..64
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 tctctggcta actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta
       61 tagggagacc caagctggct agcgtttaaa cttaagcttg gtaccgagct cggatccact
      121 agtccagtgt ggtggaattc tgcagatatc cagcacagtg gcggccgcgc caccatgaag
      181 acgatcatcg ccctgagcta catcttctgc ctggtattcg ccgactacaa ggacgatgat
      241 gacgccagca tcgatatgca gttctcaggc gaaaaaattt caggacagcg agacttgcag
      301 aaatccaaaa tgcgctttac atttacatcc agatgtctcg cactgttcct ccttcttaat
      361 catcccacac ccatcctgcc tgccttttca aatcagacat accctaccat cgaaccaaaa
      421 ccctttttgt atgttgtggg acgaaaaaag atgatggacg ctcagtacaa gtgctatgat
      481 aggatgcaac aactgccggc gtaccaggga gagggcccat attgtaacag aacctgggac
      541 gggtggctgt gctgggacga tactcccgct ggtgtccttt cctaccaatt ctgccctgat
      601 tatttcccgg actttgaccc aagcgagaaa gtgaccaaat attgcgacga aaaaggcgtc
      661 tggtttaaac acccggagaa taaccggacg tggtcaaact ataccatgtg caacgcgttt
      721 acccccgaaa agcttaaaaa cgcttacgtc ctttattatc tggctattgt gggacattct
      781 ctgagtattt tcacactcgt gattagcttg ggcatattcg tcttcttcag gaaacttacc
      841 actatatttc cactgaactg gaagtatcga aaagccctga gcctggggtg tcagagagta
      901 acccttcaca agaatatgtt ccttacctat atccttaact ccatgatcat tattattcat
      961 ctggtggaag tcgtgccaaa tggtgaactg gtgcgcaggg atccggtgtc ctgcaagatt
     1021 ctgcatttct ttcaccagta tatgatggca tgcaattact tctggatgct gtgcgaaggg
     1081 atctacctgc acactcttat cgt
//
