LOCUS       sequence-117024-         864 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd gen lentiviral backbone for cloning and expression of new shRNA 
            sequences. Uses hygromycin for selection..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 864)
  TITLE     empty vector
REFERENCE   2  (bases 1 to 864)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pLKO.1 hygro
FEATURES             Location/Qualifiers
     source          1..864
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    88..205
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        254..764
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     primer_bind     683..701
                     /label=hPGK-F
                     /note="Human PGK promoter, forward primer"
ORIGIN
        1 tatcttgtgg aaaggacgaa acaccggtcc gcaggtatgc acgcgtgaat tctcgacctc
       61 gagacaaatg gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag
      121 tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag aattacaaaa
      181 acaaattaca aaaattcaaa attttcgggt ttattacagg gacagcagag atccactttg
      241 gccgcggctc gagggggttg gggttgcgcc ttttccaagg cagccctggg tttgcgcagg
      301 gacgcggctg ctctgggcgt ggttccggga aacgcagcgg cgccgaccct gggtctcgca
      361 cattcttcac gtccgttcgc agcgtcaccc ggatcttcgc cgctaccctt gtgggccccc
      421 cggcgacgct tcctgctccg cccctaagtc gggaaggttc cttgcggttc gcggcgtgcc
      481 ggacgtgaca aacggaagcc gcacgtctca ctagtaccct cgcagacgga cagcgccagg
      541 gagcaatggc agcgcgccga ccgcgatggg ctgtggccaa tagcggctgc tcagcagggc
      601 gcgccgagag cagcggccgg gaaggggcgg tgcgggaggc ggggtgtggg gcggtagtgt
      661 gggccctgtt cctgcccgcg cggtgttccg cattctgcaa gcctccggag cgcacgtcgg
      721 cagtcggctc cctcgttgac cgaatcaccg acctctctcc ccagggggat ccaccggatc
      781 aagcttgcca gctggggcgc cctctggtaa ggttgggaag ccctgcagat ctgatcccgg
      841 ggggcaatga gatatgaaaa agcc
//
