LOCUS       sequence-117029-        1081 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of G protein-coupled receptors for PRESTO-Tango: parallel
            receptorome expression and screening via transcriptional output, 
            with transcriptional activation following arrestin translocation.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1081)
  AUTHORS   Kroeze WK, Sassano MF, Huang XP, Lansu K, McCorvy JD, Giguere PM, 
            Sciaky N, Roth BL
  TITLE     PRESTO-Tango as an open-source resource for interrogation of the 
            druggable human GPCRome.
  JOURNAL   Nat Struct Mol Biol. 2015 May;22(5):362-9. doi: 10.1038/nsmb.3014. 
            Epub 2015 Apr 20.
  PUBMED    25895059
REFERENCE   2  (bases 1 to 1081)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nsmb.3014";
            journalName: "Nat Struct Mol Biol"; date: "2015-05"; volume: "22"; 
            issue: "5"; pages: "362-9"
COMMENT     Sequence Label: FFA2-Tango
FEATURES             Location/Qualifiers
     source          1..1081
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     46..65
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        46..64
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      1009..1018
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 tctctggcta actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta
       61 tagggagacc caagctggct agcgtttaaa cttaagcttg gtaccgagct cggatccact
      121 agtccagtgt ggtggaattc tgcagatatc cagcacagtg gcggccgcgc caccatgaag
      181 acgatcatcg ccctgagcta catcttctgc ctggtattcg ccgactacaa ggacgatgat
      241 gacgccagca tcgatatgct tccagactgg aaatcttccc tgatactgat ggcctacata
      301 attattttcc ttacaggtct ccctgctaat ctgctggccc tgagggcttt tgtcggcagg
      361 atccgccagc cccaacccgc ccctgtccac atcctcttgc tgtccctgac cctggcggat
      421 ctcctgttgc tgcttcttct cccttttaag attattgaag ccgcttccaa tttcaggtgg
      481 tacttgccca aggtggtatg cgcccttacc tcctttggct tctacagtag catctactgc
      541 tccacttggc ttttggcagg aatctccatt gagcgctatc tcggcgtagc attccccgtg
      601 cagtacaagc tctcacgccg ccctctctat ggagtgatag ctgcactcgt ggcttgggtt
      661 atgagtttcg gccattgcac catcgtgatt atcgtacagt atctgaatac caccgagcag
      721 gtgaggagcg ggaacgaaat aacatgttac gagaatttta cggataatca gctcgacgtc
      781 gttttgccgg ttcggctgga actgtgcttg gtgctgttct tcatcccaat ggcagttacc
      841 atcttttgtt attggagatt tgtgtggatc atgctttccc aacctttggt aggggcacag
      901 agaaggcgcc gagctgttgg attggctgtt gttaccctgc tgaatttcct cgtgtgcttt
      961 ggcccatata atgtgagcca cctggttggc tatcatcagc gcaagtcccc atggtggcgc
     1021 agcatagccg tggtgttttc cagcctcaat gcaagcctgg atcctctcct gttctacttc
     1081 a
//
