LOCUS       sequence-117032-         945 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  gltA targeting guide RNA E. coli , Low Phosphate Induction.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 945)
  TITLE     SUPERSEDE
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 945)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pCASCADE-gltA2
FEATURES             Location/Qualifiers
     source          1..945
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     174..191
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(585..612)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(628..699)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(784..803)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
ORIGIN
        1 cactgatttg agcgtcagat ttcgtgatgc ttgtcagggg ggcggagcct atggaaaaac
       61 ggctttgccg cggccctctc acttccctgt taagtatctt cctggcatct tccaggaaat
      121 ctccgccccg ttcgtaagcc atttccgctc gccgcagtcg aacgaccgag cgtagcgagt
      181 cagtgagcga ggaagcggaa tatatcccta ggaaaaaaaa ccccgccctg tcaggggcgg
      241 ggtttttttt tcggtttatc cccgctggcg cggggaactc gaactaataa ctgtcccgaa
      301 tgaattggtc aatacggttt atccccgctg gcgcggggaa ctcgaggtgg taccagatct
      361 tttgaattca aagttaaaca aaattatttg tagagggaaa ccgttgtggt ctccctgaat
      421 atacgcgttt tatgctcgcg gggtaacatg acatgctcga attacagaaa aataactttt
      481 ttgttacatt tgtaagatag taaggtgtca gaaagagtta tccagcaacc actcatagac
      541 ctagggcagc agatagggac gacgtggtgt tagctgtgta taaacgcaga aaggcccacc
      601 cgaaggtgag ccagtgtgac tctagtagag agcgttcacc gacaaacaac agataaaacg
      661 aaaggcccag tctttcgact gagcctttcg ttttatttga tgcctggatg atgtctagat
      721 tagataaaag taaagtgatt aacagcgcat tagagctgct taatgaggtc ggaatcgaag
      781 gtttaacaac ccgtaaactc gcccagaagc taggtgtaga gcagcctaca ttgtattggc
      841 atgtaaaaaa taagcgggct ttgctcgacg ccttagccat tgagatgtta gataggcacc
      901 atactcactt ttgcccttta gaaggggaaa gctggcaaga ttttt
//
