LOCUS       sequence-117060-         915 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses EGFP-tagged MTR-3 in Drosophila cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 915)
  TITLE     Izaurralde lab Exosc plasmid
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 915)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pAc5.1B-EGFP-DmMTR-3
FEATURES             Location/Qualifiers
     source          1..915
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     33..50
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(73..90)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(100..141)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 ctcgatcgag gctgatcagc gagctcaggc cttagaaggc acagtcgagg ctgatcagcg
       61 ggtttaaact caatggtgat ggtgatgatg accggtacgc gtagaatcga gaccgaggag
      121 agggttaggg ataggcttac cttcgaaccg cgggccctct agactcgagc tactgcctct
      181 ttacataggg atcctcccgg atgttcgatt gtataaactc ctctggtcct tgcttcttta
      241 gcttctcgat tatttcctcc aaacgctgat cttctagagc ggtctcggct ttctcgcgta
      301 gatctagttc gtgctgctcc ttcacccgct tcgtgagcac gcctttgact agttctctca
      361 gggacttgtt aatggccagg gtgtaatcca gcagctttac ataggtggct ggactgagat
      421 atccgcactg ctggcactgg gcgatctgtt caaaggtatc catgctggcg gtaatgatca
      481 ggccgtgctc ctgggcggat gacgggctgg ttgtgctatc ggtgctgctg ttgcgatgct
      541 tccagagcag ctcctcaacc ttggcactgg gattcaggaa gacgtgatcc cgatagatac
      601 aagctgtgga ggccgtaatt aagtcgtaag tggaaatgcc gcactccacc aaagcgactc
      661 cgcagcaatt gatggcagtg ctgagcaggc agccgtcatc gtcgagaata agcacccgta
      721 tgtccagctg aaagttaagg aactccgtgc ggcacaccac cggctccatg gccttggtca
      781 gcatgctgct caggtggcgt tcccgttccg gcaccgaatc tagatcccct gtgctgaagg
      841 ccgcaaaatt cacatagcaa ttcagaacac ccatgttcat tctgcgtgcg ctggcgcgga
      901 ttagctcctt tggcg
//
