LOCUS       sequence-117067-         853 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses EGFP-tagged TRF4-2 in Drosophila cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 853)
  TITLE     Izaurralde lab Exosc plasmid
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 853)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pAc5.1B-EGFP-DmTRF4-2
FEATURES             Location/Qualifiers
     source          1..853
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     33..50
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(73..90)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(100..141)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 ctcgatcgag gctgatcagc gagctcaggc cttagaaggc acagtcgagg ctgatcagcg
       61 ggtttaaact caatggtgat ggtgatgatg accggtacgc gtagaatcga gaccgaggag
      121 agggttaggg ataggcttac cttcgaaccg cgggccctct agactcgagt taattcatct
      181 taaggtttgc aaggtcggca actatgtcta tgttttgggg aggatcatca catcggccga
      241 atccaatagt agccggcccg cccgagcgct catcctcgga ggcggaggcg gaggcggagg
      301 gggatgtgga tcccgtaggc gacggttgta caagggaatc ggcacaaggc aattccggtg
      361 tcaaaaggtg ctggaagttg tagtgcaccc aagcccggta gttgatgatg gactgcggca
      421 cctctacaat gttggccagt atggggccga cgatcttgga cgagtcggag tccaccagct
      481 tcgagagctt cacgaaggct gccccgaatc cctgcatcac gccgagcacc ccataggagc
      541 tcctccctat gtcgttggtc ggcgtcactg gatcctctat gcaaagcacc gactgccagt
      601 tgttctcgcc gagcgtggag cgaagtcgag ccttctccac gcaaccgcct tggccgagga
      661 cagaaattcc atacttgaag aagtcaaact tgcggccgta gtagtctaga aactgaatga
      721 gcagcagggc cagcttactg tgttcactta aacgcctgtt ggagcgcgca tgctgctgca
      781 gaaagctgat caccattagc gtgagtgcgt aggacgagac tccgccggag ttgtagacct
      841 cgttgaatcc gtg
//
