LOCUS       sequence-117073-         475 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses EGFP-tagged MTR-3 in Drosophila cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 475)
  TITLE     Izaurralde lab Exosc plasmid
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 475)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pAc5.1B-EGFP-DmMTR-3
FEATURES             Location/Qualifiers
     source          1..475
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(2..4,5..7,8..466)
                     /codon_start=1
                     /product="improved N-terminal fragment of mVenus for use in
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN155(I152L)
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMA"
     primer_bind     complement(47..68)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(308..327)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga
       61 cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta
      121 cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac
      181 cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg accacatgaa
      241 gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt
      301 cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct
      361 ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca
      421 caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca agcag
//
