LOCUS       sequence-117079-         785 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses CAS9 nuclease in mammalian cells. Used to modify genome of
            mouse embroys.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 785)
  AUTHORS   Fujii W, Kawasaki K, Sugiura K, Naito K
  TITLE     Efficient generation of large-scale genome-modified mice using gRNA 
            and CAS9 endonuclease.
  JOURNAL   Nucleic Acids Res. 2013 Aug 30.
  PUBMED    23997119
REFERENCE   2  (bases 1 to 785)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2013 Aug 30."
COMMENT     Sequence Label: pCAG-T3-hCAS-pA
FEATURES             Location/Qualifiers
     source          1..785
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(96..107)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 atcgaacttc cgttgtgtga tcagtttggc gttcagcagc tgccgccaat aatttttcat
       61 tttcttgaca acttcttctg aggggacgtt atcactcttc cctctatttt tatcggatct
      121 tgtcaacact ttattatcaa tagaatcatc tttgagaaaa gactggggca cgatatgatc
      181 cacgtcgtag tcggagagcc gattgatgtc cagttcctga tccacgtaca tgtccctgcc
      241 gttctgcagg tagtacaggt agagcttctc attctgaagc tgggtgtttt caactgggtg
      301 ttccttaagg atttgggacc ccagttcttt tataccctct tcaatcctct tcatcctttc
      361 cctactgttc ttctgtccct tctgggtagt ttggttctct cgggccatct cgataacgat
      421 attctcgggc ttatgccttc ccattacttt gacgagttca tccacgacct taacggtctg
      481 cagtattccc tttttgatag ctgggctacc tgcaagatta gcgatgtgct cgtgaagact
      541 gtccccctgg ccagaaactt gtgctttctg gatgtcctcc ttaaaggtga gagagtcatc
      601 atggatcaac tgcatgaagt tccggttggc aaatccatcg gacttaagaa aatccaggat
      661 tgtctttcca ctctgcttgt ctcggatccc attgatcagt tttcttgaca gccgccccca
      721 tcctgtatat cggcgcctct tgagctgttt catgactttg tcgtcgaaga gatgagcgta
      781 agttt
//
