LOCUS       sequence-117085-         350 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses CAS9 nuclease in mammalian cells. Used to modify genome of
            mouse embroys.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 350)
  AUTHORS   Fujii W, Kawasaki K, Sugiura K, Naito K
  TITLE     Efficient generation of large-scale genome-modified mice using gRNA 
            and CAS9 endonuclease.
  JOURNAL   Nucleic Acids Res. 2013 Aug 30.
  PUBMED    23997119
REFERENCE   2  (bases 1 to 350)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2013 Aug 30."
COMMENT     Sequence Label: pCAG-T3-hCAS-pA
FEATURES             Location/Qualifiers
     source          1..350
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             53..76
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             86..106
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 gctggagctc caccgcggtg gcggccgctc tagcccgggc ggatccacca tggattacaa
       61 ggatgacgac gataagatca tggccccaaa gaagaagcgg aaggtcggta tccacggagt
      121 cccagcagcc gacaagaagt actccattgg gctcgatatc ggcacaaaca gcgtcggctg
      181 ggccgtcatt acggacgagt acaaggtgcc gagcaaaaaa ttcaaagttc tgggcaatac
      241 cgatcgccac agcataaaga agaacctcat tggcgccctc ctgttcgact ccggggagac
      301 ggccgaagcc acgcggctca aaagaacagc acggcgcaga tatacccgca
//
