LOCUS       sequence-117087-         716 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses EGFP-tagged RBM7 in Drosophila cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 716)
  TITLE     Izaurralde lab Exosc plasmid
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 716)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pAc5.1B-EGFP-DmRBM7
FEATURES             Location/Qualifiers
     source          1..716
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(2..4,5..7,8..715)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELY"
     CDS             join(2..4,5..7,8..715)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELY"
     primer_bind     complement(47..68)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(308..327)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     655..676
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 catggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg tcgagctgga
       61 cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg atgccaccta
      121 cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc cctggcccac
      181 cctcgtgacc accctgacct acggcgtgca gtgcttcagc cgctaccccg accacatgaa
      241 gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc gcaccatctt
      301 cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg gcgacaccct
      361 ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca tcctggggca
      421 caagctggag tacaactaca acagccacaa cgtctatatc atggccgaca agcagaagaa
      481 cggcatcaag gtgaacttca agatccgcca caacatcgag gacggcagcg tgcagctcgc
      541 cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc ccgacaacca
      601 ctacctgagc acccagtccg ccctgagcaa agaccccaac gagaagcgcg atcacatggt
      661 cctgctggag ttcgtgaccg ccgccgggat cactctcggc atggacgagc tgtaca
//
