LOCUS       sequence-117093-        4454 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of Red fluorescent genetically encoded Ca2+ 
            indicator for optical imaging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4454)
  AUTHORS   Zhao Y, Araki S, Wu J, Teramoto T, Chang YF, Nakano M, Abdelfattah 
            AS, Fujiwara M, Ishihara T, Nagai T, Campbell RE
  TITLE     An Expanded Palette of Genetically Encoded Ca2+ Indicators.
  JOURNAL   Science. 2011 Sep 30;333(6051):1888-91. doi: 10.1126/science.1208592
  PUBMED    21903779
REFERENCE   2  (bases 1 to 4454)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2011-09-30- 30"; volume: "333"; 
            issue: "6051"; pages: "1888-91"
COMMENT     Sequence Label: CMV-R-GECO1
FEATURES             Location/Qualifiers
     source          1..4454
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    42..266
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     255..272
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     promoter        292..310
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     292..309
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     CDS             complement(371..814)
                     /codon_start=1
                     /product="calcium-binding protein"
                     /label=calmodulin
                     /translation="DQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEA
                     ELQDMINEVDADGDGTFDFPEFLTMMARKMNDTDSEEEIREAFRVFDKDGNGYIGAAEL
                     RHVMTDLGEKLTDEEVDEMIRVADIDGDGQVNYEEFVQMMTAK"
     primer_bind     complement(838..857)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     CDS             complement(1553..1609)
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKAGHAVRAIGRLSS"
     primer_bind     complement(1658..1677)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1659..1677)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(1722..1925)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(1751..1771)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        1926..2305
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     2477..2496
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        2571..2675
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2676..2744,2745..3536)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2894..2913)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3707..4295
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4196..4215
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttccggacta gagccccagc tggttctttc cgcctcagaa gccatagagc ccaccgcatc
       61 cccagcatgc ctgctattgt cttcccaatc ctcccccttg ctgtcctgcc ccaccccacc
      121 ccccagaata gaatgacacc tactcagaca atgcgatgca atttcctcat tttattagga
      181 aaggacagtg ggagtggcac cttccagggt caaggaaggc acgggggagg ggcaaacaac
      241 agatggctgg caactagaag gcacagtcga ggctgatcag cgagctctag catttaggtg
      301 acactataga atagggccct ctagatgcat gctcgagcgg ccgccagtgt gatggatatc
      361 tgcagaattc ctacttcgct gtcatcattt gtacaaactc ttcgtagttt acctgaccat
      421 ccccatcgat gtctgctacc ctgatcattt catcaacctc ctcatctgtt aacttctctc
      481 caaggtctgt catcacgtgg cgaagctctg ctgcgccgat gtagccattg ccgtccttat
      541 caaacacgcg gaacgcttct ctaatttcct cttcactgtc tgtgtcattc atttttcttg
      601 ccatcatcgt caggaactca gggaagtcga atgtgccgtc accgtcggca tctacttcat
      661 tgatcatgtc ctgcagctct gcttctgtgg ggttctgccc cagagaccgc atcaccgtcc
      721 ccagctcctt ggttgttatc gtcccatccc cgtccttgtc aaatagggag aaagcctctt
      781 taaattctgc gatctgctct tcagtcagtt ggtcacgcgt agcctcccag cccatggtct
      841 tcttctgcat tacggggccg tcggggggga agttggtgcc gcgcagcttc accttgtaga
      901 tgaatacgcc gtcctgcagg gaggagtcct ggttaacgtg aataatgccg ccgtcctcga
      961 agttcatcac gcgctcccac ctgaagccct cggggaagga cagcttgaag tagtcgggga
     1021 tgtcggctgg gtgcttaatg taggccttgg agccgtacat gaactgaggg gacaggatgt
     1081 cccaggcgaa gggcaggggg ccacccttgg tcaccttcag cttagcggtc tgaaaggcct
     1141 cgtaggggcg gccctcgccc tcgccctcga tctcgaactc gtggccgttc acggagccct
     1201 ccatgtgcac cttgaagcgc atgaactcct tgatgatggc catgttatcc tcctcgccct
     1261 tgctcaccag actcccgcct gtacctccct tgtacagctc gtccatgccg ccggtggagt
     1321 ggcggccctc ggcgcgttcg cactgttcca cgatggtgta gtcctcgttg tgggacacga
     1381 tgtccaactt gatgtcgacg atgtaggcgc cgggcagctg cacgggcttc ttggccttgt
     1441 aggtggtctt gacctcggcg gcgtagtggc cgccgtcctt cagcctcagc cccttcttga
     1501 tctcgctctt cagggcgccg tcctcggggt acatccgctc ggaaaccacg ggtgagctca
     1561 gccgacctat agctctgact gcgtgacctg ccttattcca cttacgacgt gatgagtcga
     1621 ccatggtgga tccgagctcg gtaccaagct tgggtctccc tatagtgagt cgtattaatt
     1681 tcgataagcc agtaagcagt gggttctcta gttagccaga gagctctgct tatatagacc
     1741 tcccaccgta cacgcctacc gcccatttgc gtcaatgggg cggagttgtt acgacatttt
     1801 ggaaagtccc gttgattttg gtgccaaaac aaactcccat tgacgtcaat ggggtggaga
     1861 cttggaaatc cccgtgagtc aaaccgctat ccacgcccat tgatgtactg ccaaaaccgc
     1921 atcaccatgg taatagcgat gactaatacg tagatgtact gccaagtagg aaagtcccat
     1981 aaggtcatgt actgggcata atgccaggcg ggccatttac cgtcattgac gtcaataggg
     2041 ggcgtacttg gcatatgata cacttgatgt actgccaagt gggcagttta ccgtaaatac
     2101 tccacccatt gacgtcaatg gaaagtccct attggcgtta ctatgggaac atacgtcatt
     2161 attgacgtca atgggcgggg gtcgttgggc ggtcagccag gcgggccatt taccgtaagt
     2221 tatgtaacgc ggaactccat atatgggcta tgaactaatg accccgtaat tgattactat
     2281 taataactag tcaataatca atgtcaacgc gtatatctgg cccgtacatc gcgaagcagc
     2341 gcaaaacgcc taaccctaag cagattcttc atgcaattgt cggtcaagcc ttgccttgtt
     2401 gtagcttaaa ttttgctcgc gcactactca gcgacctcca acacacaagc agggagcaga
     2461 tactggctta actatgcggc atcagagcag attgtactga gagtgcacca taggggatcg
     2521 ggagatctcc cgatccgtcg acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
     2581 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     2641 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     2701 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     2761 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     2821 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     2881 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
     2941 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     3001 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     3061 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     3121 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     3181 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
     3241 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     3301 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     3361 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     3421 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     3481 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     3541 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     3601 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     3661 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     3721 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     3781 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     3841 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     3901 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     3961 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     4021 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     4081 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     4141 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     4201 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     4261 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     4321 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     4381 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     4441 ccgagcgcag cgag
//
