LOCUS       sequence-117094-         916 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses EGFP-tagged RBM7 in Drosophila cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 916)
  TITLE     Izaurralde lab Exosc plasmid
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 916)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pAc5.1B-EGFP-DmRBM7
FEATURES             Location/Qualifiers
     source          1..916
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     33..50
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(73..90)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(100..141)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 ctcgatcgag gctgatcagc gagctcaggc cttagaaggc acagtcgagg ctgatcagcg
       61 ggtttaaact caatggtgat ggtgatgatg accggtacgc gtagaatcga gaccgaggag
      121 agggttaggg ataggcttac cttcgaaccg cgggccctct agactcgagt taaagcaatc
      181 tgcgtttgtt gttggagcgt tggtccgatc ttctgctgcc tccctgcatg tgctggtggt
      241 gttgctgtgg ttttagccgg ttgcgttcca ttacggagct gtcgctccgg cgccgttgat
      301 cggcgttgtg tccaaaagga ttgcgatcgt acggcttgcc gttatgcaaa ctgtgtcggg
      361 cgtgacgcag tggcgatggc tgcggtagag cctccatcat gaactgattg cgcagacgac
      421 tttggttgta ggcaggtagc tgcttgccgc cctgctgctt gatggtgact ttcttttgga
      481 acagttcaag gccctggtac aagtctaggg caaaaggcac cgcacacagg cgttggtatg
      541 tgacaaaccc gaaattccgg ggacgcccat tgttatcggt cggtatccgc actccttcga
      601 tggggccagc ttgcaggaac acctcgtaaa ggatctcctc cgtcacgcgc tcgtcgagat
      661 tcccgcagaa cagagtccgt tgctcctcgt cctcctcctc atcgtcgtcg tcctccagtt
      721 gcccaaccaa ctggtcaccg ttttcattgg gcaggaaagg gtaaattggc tgctcttggt
      781 gctggccaga tggaataccg tatctgtaac catctatgtt cgtttccgaa atcgtcatga
      841 ctgacgcacc actatacatt tgctgcatta gtaactgcaa gtccatcaag cttggcttgt
      901 acagctcgtc catgcc
//
