LOCUS       sequence-117095-        4448 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian expression of blue-green emission ratiometric genetically 
            encoded Ca2+ indicator for optical imaging.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4448)
  AUTHORS   Zhao Y, Araki S, Wu J, Teramoto T, Chang YF, Nakano M, Abdelfattah 
            AS, Fujiwara M, Ishihara T, Nagai T, Campbell RE
  TITLE     An Expanded Palette of Genetically Encoded Ca2+ Indicators.
  JOURNAL   Science. 2011 Sep 30;333(6051):1888-91. doi: 10.1126/science.1208592
  PUBMED    21903779
REFERENCE   2  (bases 1 to 4448)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2011-09-30- 30"; volume: "333"; 
            issue: "6051"; pages: "1888-91"
COMMENT     Sequence Label: CMV-GEM-GECO1
FEATURES             Location/Qualifiers
     source          1..4448
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    42..266
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     255..272
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     promoter        292..310
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     292..309
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     930..949
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     complement(1313..1334)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             complement(1547..1603)
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKTGHAVRAIGRLSS"
     primer_bind     complement(1652..1671)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1653..1671)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(1716..1919)
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     complement(1745..1765)
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     enhancer        1920..2299
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     primer_bind     2471..2490
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     promoter        2565..2669
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2670..2738,2739..3530)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2888..2907)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3701..4289
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4190..4209
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ttccggacta gagccccagc tggttctttc cgcctcagaa gccatagagc ccaccgcatc
       61 cccagcatgc ctgctattgt cttcccaatc ctcccccttg ctgtcctgcc ccaccccacc
      121 ccccagaata gaatgacacc tactcagaca atgcgatgca atttcctcat tttattagga
      181 aaggacagtg ggagtggcac cttccagggt caaggaaggc acgggggagg ggcaaacaac
      241 agatggctgg caactagaag gcacagtcga ggctgatcag cgagctctag catttaggtg
      301 acactataga atagggccct ctagatgcat gctcgagcgg ccgccagtgt gatggatatc
      361 tgcagaattc ctacttcgct gtcatcattt gtacaaactc ttcgtagttt acctgaccat
      421 ccccatcgat gtctgcaacc ctgatcattt catcaacctc ttcatctgtt aacttctctc
      481 caaggtttgt catcacgtgg cgaagctctg ctgcgccgat gtagccattg ccgtccttat
      541 caaacacacg gaacgcttct ctaatttctt cttcactgtc tgtgtcctgc attttaggtg
      601 ccatcattgt caggaactca gggaagtcga ttgtgccgtc accgtcggca tctacttcat
      661 tgatcatgtc ctgcagctct gcttctgtgg ggttctgccc cagagaccgc atcaccgtcc
      721 ccagctcctt ggttgttatc gtcccatccc cgtccttgtc aaatagggag aaagcctctt
      781 taaattctgc gatctgctct tcagtcagtt ggtcacgcgt gctgtactcc agcttgtgcc
      841 ccaggatgtt gccgtcctcc ttgaagtcga tgcccttcag ctcgatgcgg ttcaccaggg
      901 tgtcgccctc gaacttcacc tcggcgcggg tcttgtagtt gccgtcgtcc ttgaagaaga
      961 tggtgcgctc ctggatgtag ccttcgggca tggcggactt gaagaagtcg tgctgcttca
     1021 tgtggtcggg gtagcggctg aagcactgca cgccgtagga cagggtggtc acgagggtgg
     1081 gccagggcac gggcagcttg ccggtggtgc agatgaactt cagggtcagc ttgccgtagg
     1141 tggcatcgcc ctcaccctcg ccggacacgc tgaacttgtg gccgtttacg tcgccgtcca
     1201 gctcgacctg gatgggcacc accccggtga acagctcctc gcccttgctc accatgccac
     1261 cgctgccacc cttgtacagc tcgtccatgc cgagagtgat cccggcggcg gtcacgaact
     1321 ccagcaggac catgtgatcg cgcttctcgt tggggtcttt cgaaagtatg gactgcacgc
     1381 tcaggtagtg gttgtcgggc agcagcacgg ggccgtcgcc tatgggggtg atctgctggt
     1441 agtggtaggc gagctgcacg ccgccgccct cgatgttgtg gcggatcttg aagtacgcct
     1501 tgatgccgtt cttctgctcg tcggccttta tatacacgtt ctctggtgag ctcagccgac
     1561 ctatagctct gactgcgtga cctgtcttat tccacttacg acgtgatgag tcgaccatgg
     1621 tggatccgag ctcggtacca agcttgggtc tccctatagt gagtcgtatt aatttcgata
     1681 agccagtaag cagtgggttc tctagttagc cagagagctc tgcttatata gacctcccac
     1741 cgtacacgcc taccgcccat ttgcgtcaat ggggcggagt tgttacgaca ttttggaaag
     1801 tcccgttgat tttggtgcca aaacaaactc ccattgacgt caatggggtg gagacttgga
     1861 aatccccgtg agtcaaaccg ctatccacgc ccattgatgt actgccaaaa ccgcatcacc
     1921 atggtaatag cgatgactaa tacgtagatg tactgccaag taggaaagtc ccataaggtc
     1981 atgtactggg cataatgcca ggcgggccat ttaccgtcat tgacgtcaat agggggcgta
     2041 cttggcatat gatacacttg atgtactgcc aagtgggcag tttaccgtaa atactccacc
     2101 cattgacgtc aatggaaagt ccctattggc gttactatgg gaacatacgt cattattgac
     2161 gtcaatgggc gggggtcgtt gggcggtcag ccaggcgggc catttaccgt aagttatgta
     2221 acgcggaact ccatatatgg gctatgaact aatgaccccg taattgatta ctattaataa
     2281 ctagtcaata atcaatgtca acgcgtatat ctggcccgta catcgcgaag cagcgcaaaa
     2341 cgcctaaccc taagcagatt cttcatgcaa ttgtcggtca agccttgcct tgttgtagct
     2401 taaattttgc tcgcgcacta ctcagcgacc tccaacacac aagcagggag cagatactgg
     2461 cttaactatg cggcatcaga gcagattgta ctgagagtgc accatagggg atcgggagat
     2521 ctcccgatcc gtcgacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     2581 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     2641 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     2701 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     2761 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     2821 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     2881 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     2941 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     3001 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     3061 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     3121 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     3181 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     3241 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     3301 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     3361 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     3421 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     3481 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     3541 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     3601 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     3661 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     3721 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     3781 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     3841 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     3901 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     3961 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     4021 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     4081 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     4141 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     4201 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     4261 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     4321 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     4381 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     4441 gcagcgag
//
