LOCUS       sequence-117102-         754 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of the mutated N terminal part (R5A-K5A N23, 
            transmembrane part of 23 aa) of C11orf83/UQCC3 fused to GFP protein 
            (C-ter).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 754)
  AUTHORS   Desmurs M, Foti M, Raemy E, Vaz FM, Martinou JC, Bairoch A, Lane L
  TITLE     C11orf83, a mitochondrial cardiolipin-binding protein involved in 
            bc1 complex assembly and supercomplex stabilization.
  JOURNAL   Mol Cell Biol. 2015 Apr;35(7):1139-56. doi: 10.1128/MCB.01047-14. 
            Epub 2015 Jan 20.
  PUBMED    25605331
REFERENCE   2  (bases 1 to 754)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/MCB.01047-14"; journalName: "Mol Cell Biol"; date: 
            "2015-04"; volume: "35"; issue: "7"; pages: "1139-56"
COMMENT     Sequence Label: pcDNA-Dest47- R5A-K5A N23 C11orf83-GFP
FEATURES             Location/Qualifiers
     source          1..754
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     50..69
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        50..68
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    101..125
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     RBS             137..157
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    complement(252..276)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     primer_bind     complement(320..348)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
       61 actataggga gacccaagct ggctagttaa gcttgatcaa acaagtttgt acaaaaaagc
      121 aggctccgcg gccgccttgt ttaactttaa gaaggagccc ttcaccatgg attccttggc
      181 ggcaatgctg atctcagtcg caatgctggg cgcaggggct ggcgtgggct acgcgaaggg
      241 tgggcgcgcc gacccagctt tcttgtacaa agtggttcga tctagaatgg ctagcaaagg
      301 agaagaactt ttcactggag ttgtcccaat tcttgttgaa ttagatggtg atgttaatgg
      361 gcacaaattt tctgtcagtg gagagggtga aggtgatgct acatacggaa agcttaccct
      421 taaatttatt tgcactactg gaaaactacc tgttccatgg ccaacacttg tcactacttt
      481 ctcttatggt gttcaatgct tttcccgtta tccggatcat atgaaacggc atgacttttt
      541 caagagtgcc atgcccgaag gttatgtaca ggaacgcact atatctttca aagatgacgg
      601 gaactacaag acgcgtgctg aagtcaagtt tgaaggtgat acccttgtta atcgtatcga
      661 gttaaaaggt attgatttta aagaagatgg aaacattctc ggacacaaac tcgagtacaa
      721 ctataactca cacaatgtat acatcacggc agac
//
